猪DDX家族成员基因结构与表达特性分析  

Analysis of gene structure and expression characteristics of pig DDX family members

作　　者：席梦瑶 王舒 马艺珈 郭晓红[1] 曹果清[1] 李步高[1] 高鹏飞[1] XI Mengyao;WANG Shu;MA Yijia;GUO Xiaohong;CAO Guoqing;LI Bugao;GAO Pengfei(College of Animal Science,Shanxi Agricultural University,Jinzhong 030801,China)

机构地区：[1]山西农业大学动物科学学院,山西晋中030801

出　　处：《黑龙江畜牧兽医》2023年第3期52-57,133-135,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：山西省基础研究项目(202203021211268);山西农业大学产业引领工程项目(CYYL22-13);山西农业大学大学生创新项目。

摘　　要：为了系统研究DDX解旋酶家族成员在猪生长发育中的作用,试验采用生物信息学方法和实时荧光定量PCR检验技术,对猪DDX家族的42个成员的基因结构、理化性质、功能结构域、聚类进化树、GO功能,以及在大白猪和马身猪盲肠组织中的表达情况进行了研究。结果表明:猪DDX家族除DDX28和DDX53基因没有内含子外,其他成员均含有外显子和内含子,且外显子平均数量为16个,内含子平均数量为15个。除8号染色体外,其他染色体上均有猪DDX家族成员分布。该家族成员均为疏水蛋白并且大多数不稳定,具有多个亚细胞定位,整体来看主要分布于细胞核中。猪DDX家族成员共预测到了15个保守基序,其中DDX1、DDX11、DDX19B、DDX26、DDX36、DDX39A、DDX58和DDX60基因缺失D-E-A-D保守基序,有10个保守基序出现在超过30个成员的序列中,同时不同成员有相同的基序类型和特定的基序排列顺序。聚类分析将猪DDX家族42个成员分为6大簇,其中DDX2和DDX48、DDX21和DDX50、DDX43和DDX53、DDX5和DDX17具有高度同源性,支持率为100%。该家族大多数成员具有ATP依赖性RNA解旋酶活性和ATP结合功能。DDX家族基因在猪盲肠组织中存在5种表达趋势,其中DDX18、DDX21、DDX56、DDX49、DDX47、DDX20、DDX46、DDX42、DDX39B、DDX54、DDX50、DDX31和DDX28基因在两个品种猪中表达趋势相似,1日龄时表达量最高,90日龄和180日龄表达量较低。In order to systematically investigate the role of DDX helicase family members in the growth and development of pigs,the bioinformatics method and real-time fluorescence quantitative PCR(qPCR)were used to investigate the gene structure,physical and chemical properties,protein domain,cluster evolutionary tree,GO enrichment and gene expression of 42 members of pig DDX family in the cecum of large white pigs and horse-body pigs.The results showed that all members of this family had exons and introns except for DDX28 and DDX53 genes,and the average number of exons was 16 while intron number was 15.Porcine DDX family members were distributed on all chromosomes except for chromosome 8.Most of the members of this family were unstable proteins with hydrophobic properties.Most of them had multiple subcellular localization and were mainly distributed in the nucleus.The family members predicted 15 conserved motifs,among which DDX1,DDX11,DDX19B,DDX26,DDX36,DDX39A,DDX58 and DDX60 lacked D-E-A-D conserved motifs,and the 10 conservative base sequences were found in the sequences of more than 30 members.At the same time,different members often had the same base sequence type and specific base sequence order.The 42 members of porcine DDX family were divided into 6 clusters by cluster analysis,among which DDX2 and DDX48,DDX21 and DDX50,DDX43 and DDX53,and DDX5 and DDX17 had high homology and the support rate was 100%.Most members of this family had ATP-dependent RNA helicase activity and ATP-binding function.There were 5 expression trends of DDX family genes in porcine caecum,among which DDX18,DDX21,DDX56,DDX49,DDX47,DDX20,DDX46,DDX42,DDX39B,DDX54,DDX50,DDX31 and DDX28 genes showed similar expression trends in the two cultivars.The expression of these genes was the highest at 1 day of age,while the expression level was lower at 90 and 180 day.

关 键 词：猪 DDX家族 功能结构域 生物信息学分析 表达特性 

分 类 号：S813.3[农业科学—畜牧学]