不同精粗比日粮对湖羊血清酶活性、免疫性能和肌肉成分的影响  

Effects of different concentrate to forage diets on serum enzyme activities,immune performance and muscle composition of Hu sheep

作　　者：刘宇航 谷志勇 王海波 王丽媛 占今舜[1] 霍俊宏[1] 赵生国[2] LIU Yuhang;GU Zhiyong;WANG Haibo;WANG Liyuan;ZHAN Jinshun;HUO Junhong;ZHAO Shengguo(Institute of Animal Science and Veterinary Medicine,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;College of Animal Science and Technology,Tianjin Agricultural University,Tianjin 300191,China)

机构地区：[1]江西省农业科学院畜牧兽医研究所,南昌330200 [2]甘肃农业大学动物科学技术学院,兰州730070 [3]天津农学院动物科技学院,天津300191

出　　处：《黑龙江畜牧兽医》2023年第3期98-103,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：江西省现代农业产业技术体系建设专项(JXARS-13-肉羊岗位);江西现代农业科研协同创新专项(JXXTCX201702-04)。

摘　　要：为了探讨不同精粗比日粮对湖羊血清酶活性、免疫性能和肌肉成分的影响,试验将体重相近、健康的40只湖羊随机分为4组,每组10只,公母各半,分别饲喂精粗比为70∶30(试验Ⅰ组)、60∶40(试验Ⅱ组)、50∶50(试验Ⅲ组)和40∶60(试验Ⅳ组)的全混合日粮。试验期为70 d。于试验第14,35,56天每组随机选取6只湖羊空腹颈静脉采集血液5 mL,用于血清酶[天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、肌酸激酶(CK)、碱性磷酸酶(ALP)、皮质醇(COR)]活性和免疫性能指标[免疫球蛋白A(IgA)、免疫球蛋白G(IgG)和免疫球蛋白M(IgM)]的测定;饲养结束后每组随机选取6只湖羊屠宰,取背最长肌用液相色谱仪测定氨基酸和脂肪酸含量。结果表明:试验第14天,试验Ⅰ组湖羊血清中ALT活性显著高于试验Ⅳ组(P<0.05),试验Ⅳ组AST/ALT显著高于试验Ⅰ组和试验Ⅱ组(P<0.05);第35天,试验Ⅰ组湖羊血清中ALT活性显著高于试验Ⅳ组(P<0.05),试验Ⅰ组和试验Ⅳ组AST/ALT显著高于试验Ⅱ组和试验Ⅲ组(P<0.05),试验Ⅰ组CK活性显著高于试验Ⅱ组和试验Ⅲ组(P<0.05),试验Ⅲ组湖羊血清中ALP活性显著高于试验Ⅰ组(P<0.05);第56天,试验Ⅳ组湖羊血清中ALT活性显著低于试验Ⅱ组(P<0.05)。试验第14天,试验Ⅲ组湖羊血清中IgG浓度显著高于试验Ⅱ组和试验Ⅳ组(P<0.05);第35天,试验Ⅳ组湖羊血清中IgG和IgA浓度显著低于试验Ⅲ组(P<0.05)。第56天,试验Ⅳ组湖羊血清中IgM浓度显著低于试验Ⅰ组(P<0.05)。各组间氨基酸总量(TAA)差异不显著(P>0.05),而试验Ⅳ组天冬氨酸、谷氨酸、丝氨酸和精氨酸含量显著高于试验Ⅲ组(P<0.05),蛋氨酸含量显著高于试验Ⅰ组(P<0.05)。各组间脂肪酸含量差异不显著(P>0.05),然而试验Ⅰ组的大部分脂肪酸含量高于其他各组。说明低精粗比(40∶60)日粮有利于提高血清酶活性、免疫性能和肌肉中部分氨基酸含量,而对肌肉脂肪In order to investigate the effects of different concentrate to forage ratio diets on serum enzyme activity,immune performance and muscle composition of Hu sheep,forty healthy Hu sheep were randomly divided into four groups with ten sheep in each group,half male and half female.The sheep in the four groups were fed a complete mixed diet with concentrate to forage ratio of 70∶30(test groupⅠ),60∶40(test groupⅡ),50∶50(test groupⅢ)and 40∶60(test groupⅣ),respectively.The experiment lasted for 70 days.On day 14,35,and 56 of the experiment,six Hu sheep in each group were randomly selected to collect 5 mL blood from the fasting jugular vein.The isolated serum was used to determine the activity of serum enzymes(aspartate aminotransferase[AST],alanine aminotransferase[ALT],creatine kinase[CK],alkaline phosphatase[ALP],cortisol[COR])and immune performance indexes(immunoglobulin A[IgA],immunoglobulin protein G[IgG]and immunoglobulin protein M[IgM]).After the feeding experiment,six Hu sheep in each group were selected and slaughtered.The longissimus dorsi muscles were collected for determination of the contents of amino acids and fatty acids by liquid chromatograph.The results showed that on the 14th day of experiment,the serum ALT activity in the test groupⅠwas significantly higher than that in the test groupⅣ(P<0.05),and the AST/ALT in the test groupⅣwas significantly higher than that in the test groupsⅠandⅡ(P<0.05).On the 35th day,the activity of ALT in serum of lake sheep in the test groupⅠwas significantly higher than that in the test groupⅣ(P<0.05),the AST/ALT in the test groupsⅠandⅣwas significantly higher than that in the test groupsⅡandⅢ(P<0.05),and the activity of CK in the test groupⅠwas significantly higher than that in the test groupsⅡandⅢ(P<0.05).The serum ALP activity of Hu sheep in the test groupⅢwas significantly higher than that in the test groupⅠ(P<0.05).On the 56th day,the serum ALT activity of Hu sheep in the test groupⅣwas significantly lower than that in

关 键 词：湖羊 全混合日粮 酶活性 免疫蛋白 肌肉成分 

分 类 号：S816.13[农业科学—饲料科学]