基于网络药理学方法探讨佳蓉片治疗男性迟发性性腺功能减退症的可能机制  

Potential mechanisms of Jiarong Tablets in the treatment of late-onset hypogonadism in men:An exploration based on network pharmacology

作　　者：胡瑞[1] 吴悔 李波男 宁港 石若冰 周兴[2] 高茗 HU Rui;WU Hui;LI Bo-nan;NING Gang;SHI Ruo-bing;ZHOU Xing;GAO Ming(Center of Health Management,Jurong Hospital Afiliated to Jiangsu University,Jurong,Jiangsu 212400,China;Department of Andrology,The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China;Department of Pharmacy,Jinling Hospital Affiliated to Nanjing University School of Medicine/General Hospital of Eastern Theater Command,Nanjing,Jiangsu 210002,China)

机构地区：[1]江苏大学附属句容医院健康管理中心,江苏句容212400 [2]湖南中医药大学第一附属医院男科,湖南长沙410007 [3]南京大学医学院附属金陵医院/东部战区总医院药剂科,江苏南京210002

出　　处：《中华男科学杂志》2022年第10期915-925,共11页National Journal of Andrology

基　　金：国家自然科学基金(82074444,81673984);湖南省教育厅重点项目(20A368);长沙市科技计划项目经费资助-长沙市杰出创新青年培养计划(kq1802015)~~。

摘　　要：目的:通过网络药理学方法研究佳蓉片治疗男性迟发性性腺功能减退症(LOH)的分子靶点和信号通路,探讨可能潜在机制。方法:应用中药系统药理学分析平台(TCMSP)获取佳蓉片活性成分和作用靶点;GeneCards、OMIM数据库获取LOH的疾病靶点;筛选出与佳蓉片共同的靶点,Venny 2.1绘制共同靶点韦恩图;STRING构建共同靶点互作网络(PPI);Cytoscape 3.7.2软件构建佳蓉片-活性成分-LOH-靶点交集的网络;R语言软件对共同靶点进行生物功能(GO)和KEGG富集分析,筛选出佳蓉片治疗LOH的可能信号通路。结果:共获得佳蓉片80个生物活性成分,64个佳蓉片治疗LOH的共同靶点,其中核心靶点依次有IL-6、INS、AKT1、JUN、MAPK8等,GO和KEGG分析,上述靶点主要涉及MAPK、HIF-1、Ras、ErbB等多条信号通路。结论:网络药理学分析,佳蓉片治疗LOH具有的多靶点、多途径、多层次特点,主要与Leydig细胞睾酮合成酶表达、氧化应激、凋亡等相关,为后续临床和基础实验验证提供了很好的理论依据。Objective:To study the therapeutic targets and related signaling pathways of Jiarong Tablets(JRT)in the treatment of late-onset hypogonadism(LOH)in males by network pharmacology,and further analyze its potential action mechanism.Methods:Using the Chinese Medicine System Pharmacology Analysis Platform(TCMSP),we obtained the active ingredients and therapeutic targets of JRT,disease targets of LOH through the GeneCards and OMIM databases,and drug-disease common targets,followed by drawing a Vennny’s diagram of the common targets.We constructed a protein-protein interaction(PPI)network of the common targets using STRING and an intersection network of JRT active ingredients-LOH-targets with Cytoscape 3.7.2,performed GO bio-functional and KEGG enrichment analyses of the common targets using the R-Language software,and identified the potential signaling pathways of JRT acting on LOH.Results:Totally,we obtained 80 bioactive ingredients from JRT and 64 common targets of LOH,with IL-6,INS,AKT1,JUN and MAPK8 as the core targets in the order of the frequency occurrences.GO and KEGG analyses showed that these targets mainly involved the MAPK,HIF-1,Ras and ErbB signaling pathways.Conclusion:JRT acts on LOH with multiple targets,through multiple routes and at multiple levels,which is related to the expression of testosterone synthetase,oxidative stress and apoptosis of Leydig cells.

关 键 词：网络药理学 佳蓉片 男性迟发性性腺功能减退症 

分 类 号：R588.1[医药卫生—内分泌] R698[医药卫生—内科学]