含明胶纳米粒的仿生关节润滑液对人工关节材料的减摩抗磨作用研究  

Antifriction and anti-wear performances of bionic lubricating fluid containing gelatin nanoparticles for artificial joint materials

作　　者：李卫华 梁风光 董诗豫 吕伟振 刘延涛 王晓 马静毅[3] LI Weihua;LIANG Fengguang;DONG Shiyu;LüWeizhen;LIU Yantao;WANG Xiao;MA Jingyi(Department of Orthopaedics,Huaihe Hospital,Henan University,Kaifeng Henan,475000,P.R.China;Henan University of Science and Technology,Luoyang Henan,471000,P.R.China;Nanoengineering Technology Center of Henan University,Kaifeng Henan,475004,P.R.China)

机构地区：[1]河南大学淮河医院骨科,河南开封475000 [2]河南科技大学,河南洛阳471000 [3]河南大学纳米工程技术中心,河南开封475004

出　　处：《中国修复重建外科杂志》2023年第2期196-201,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(51375142)。

摘　　要：目的 探讨明胶纳米粒(gelatin nanoparticle,GLN-NP)在仿生关节润滑液中对人工关节材料的减摩、抗磨作用,为发展新型仿生关节润滑液提供理论基础。方法 采用丙酮法将胶原蛋白酸性(A型)明胶经戊二醛交联得到GLN-NP,表征GLN-NP粒径大小及稳定性。将5、15、30 mg/mL GLN-NP分别与15、30 mg/mL透明质酸(hyaluronic acid,HA)复合,制备不同浓度仿生关节润滑液,并用摩擦试验机观察其对氧化锆陶瓷的减摩和抗磨作用。应用MTT法考察仿生关节润滑液各成分对RAW264.7小鼠巨噬细胞的毒性。结果 GLN-NP粒径约为139 nm,粒径分布指数为0.17,表现为单一的高峰,说明GLN-NP颗粒粒径均一。在模拟体温条件下的完全培养基、pH7.4 PBS和去离子水中,GLN-NP随时间变化的粒径变化不超过10 nm,说明GLN-NP有很好的分散稳定性,且未发生聚集现象。与单纯15、30 mg/mL HA及生理盐水相比,在其中添加不同浓度GLN-NP后,其摩擦系数明显降低,磨痕深度、宽度及磨损体积均明显减小(P<0.05);各浓度GLN-NP组间差异无统计学意义(P>0.05)。生物相容性检测示,GLN-NP、HA和HA+GLN-NP溶液随浓度提高细胞存活率稍有下降,但细胞成活率均在90%以上,组间差异无统计学意义(P>0.05)。结论 制备的含GLN-NP仿生关节润滑液具有良好的减摩、抗磨作用;其中不含HA的GLN-NP生理盐水溶液减摩、抗磨效果最好。Objective The antifriction and antiwear effects of gelatin nanoparticles(GLN-NP) on artificial joint materials in bionic joint lubricant were investigated to provide a theoretical basis for the development of new bionic joint lubricant. Methods GLN-NP was prepared by cross-linking collagen acid(type A) gelatin with glutaraldehyde by acetone method, and the particle size and stability of GLN-NP were characterized. The biomimetic joint lubricants with different concentrations were prepared by mixing 5, 15, and 30 mg/mL GLN-NP with 15 and 30 mg/mL hyaluronic acid(HA),respectively. The friction reduction and antiwear effects of the biomimetic joint lubricants on zirconia ceramics were investigated on a tribometer. The cytotoxicity of each component of bionic joint lubricant on RAW264.7 mouse macrophages was evaluated by MTT assay. Results The particle size of GLN-NP was about 139 nm, and the particle size distribution index was 0.17, showing a single peak, indicating that the particle size of GLN-NP was uniform. In complete culture medium, pH7.4 PBS, and deionized water at simulated body temperature, the particle size of GLN-NP did not change more than 10 nm with time, indicating that GLN-NP had good dispersion stability and did not aggregate.Compared with 15 mg/mL HA, 30 mg/mL HA, and normal saline, the friction coefficient, wear scar depth, width, and wear volume were significantly reduced by adding different concentrations of GLN-NP(P<0.05);there was no significant difference between different concentrations of GLN-NP(P>0.05). Biocompatibility test showed that the cell survival rate of GLN-NP, HA, and HA+GLN-NP solution decreased slightly with the increase of concentration, but the cell survival rate was more than 90%, and there was no significant difference between groups(P>0.05). Conclusion The bionic joint fluid containing GLN-NP has good antifriction and antiwear effect. Among them, GLN-NP saline solution without HA has the best antifriction and antiwear effect.

关 键 词：明胶纳米粒 仿生关节润滑液 人工关节材料 减摩 抗磨 

分 类 号：R318.08[医药卫生—生物医学工程]