哮喘患儿外周血T淋巴细胞亚群、IL-5、IL-25水平变化及其与ACT评分的相关性研究  

Changes of peripheral blood T lymphocyte subsets, interleukin-5, and interleukin-25 levels in asthma children and their correlation with Asthma Control Test score

作　　者：罗婷婷 胡梦娇 赵静利 程雨嘉 李青 LUO Ting-ting;HU Meng-jiao;ZHAO Jing-li;CHENG Yu-jia;LI Qing(Department of Pediatrics,Baoji People's Hospital,Baoji 721000,Shaanxi,CHINA)

机构地区：[1]宝鸡市人民医院儿科,陕西宝鸡721000

出　　处：《海南医学》2023年第4期553-556,共4页Hainan Medical Journal

基　　金：陕西省科学技术研究发展计划项目(编号:S2018SKZ1804591)。

摘　　要：目的 观察哮喘患儿外周血T淋巴细胞亚群、白介素-5 (IL-5)、白介素-25 (IL-25)水平,并分析其与哮喘控制测试评分表(ACT)评分的关系。方法 选取2017年5月至2020年6月宝鸡市人民医院收治的115例哮喘患儿作为观察组,依据患儿的临床表现分为缓解期组47例与急性发作期组68例,另选取同期在我院体检的50例健康儿童为对照组,检测两组儿童外周血T淋巴细胞亚群(CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))、IL-5及IL-25水平,采取ACT问卷调查患儿哮喘控制情况,并使用Pearson相关性检验法分析患儿T淋巴细胞亚群、IL-5、IL-25水平与ACT评分相关性。结果 急性发作期组患儿的CD8^(+)为(37.02±5.24)%,明显低于缓解期组的(40.19±6.23)%和对照组的(43.05±5.03)%,缓解期组又明显低于对照组,差异均有统计学意义(P<0.05);急性发作期组患儿的CD4^(+)、CD4^(+)/CD8^(+)分别为(54.78±7.06)%、1.57±0.28,明显高于缓解期组[(51.16±7.75)%、1.40±0.25]和对照组[(47.61±5.02)%、1.18±0.17],缓解期组又明显高于对照组,差异均有统计学意义(P<0.05);急性发作期组患儿的IL-5、IL-25分别为(74.92±12.13) pg/mL、(57.24±8.96) pg/mL,明显高于缓解期组[(67.38±10.54) pg/mL、(48.73±8.05) pg/mL]和对照组[(50.86±9.85) pg/mL、(44.68±7.12) pg/mL],缓解期组又明显高于对照组,差异均有统计学意义(P<0.05);急性发作期组患儿的ACT评分为(16.15±2.76)分,明显低于缓解期组的(20.84±2.37)分,差异有统计学意义(P<0.05);经Pearson相关性分析结果显示,CD8^(+)与ACT评分呈显著正相关(P<0.05),CD4^(+)、CD4^(+)/CD8^(+)、IL-5、IL-25与ACT评分呈显著负相关(P<0.05)。结论 哮喘患儿存在外周血T淋巴细胞亚群失衡、IL-5与IL-25表达水平异常升高,急性发作期变化更显著,CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)、IL-5、IL-25水平均与ACT评分密切相关,可用于评估哮喘患儿病情。Objective To observe the peripheral blood T lymphocyte subsets, interleukin-5(IL-5) and interleukin-25(IL-25) levels in asthma children, and analyze their relationship with Asthma Control Test(ACT) score.Methods A total of 115 asthma children admitted to Baoji People’s Hospital from May 2017 to June 2020 were selected as the observation group, which were divided into the remission stage group(n=47) and the acute attack group(n=68) according to clinical manifestations. In addition, 50 healthy children who were examined during the same period were selected as the control group. The levels of peripheral blood T lymphocyte subsets(CD3^(+), CD4^(+), CD8^(+)and CD4^(+)/CD8^(+)), IL-5,and IL-25 in the two groups were detected. The situation of asthma control in children was investigated by ACT. The correlation of T lymphocyte subsets, IL-5, and IL-25 levels with ACT score was analyzed by Pearson method. Results The level of CD8^(+)in the acute attack group was(37.02±5.24)%, which was significantly lower than(40.19±6.23)% in the remission stage group and(43.05±5.03)% in the control group, and the levels in the remission stage group was significantly lower than that in the control group(P<0.05). The levels of CD4^(+)and CD4^(+)/CD8^(+)in the acute attack group were(54.78±7.06)% and 1.57±0.28, which was significnatly higher than(51.16±7.75)%, 1.40±0.25 in the remission stage group and(47.61±5.02)%, 1.18±0.17 in the control group, and the levels in the remission stage group were significantly higher than in the control group(P<0.05). The levels of IL-5 and IL-25 in the acute attack group were(74.92±12.13) pg/m L and(57.24±8.96) pg/mL, which were significantly higher than(67.38±10.54) pg/mL,(48.73±8.05) pg/mL in the remission stage group and(50.86±9.85) pg/mL,(44.68±7.12) pg/mL in the control group, and the levels in the remission group were significantly higher than those in the control group(P<0.05). The ACT score in the acute attack group was(16.15±2.76) points, which was significantly lower than(20.84±

关 键 词：儿童 支气管哮喘 T淋巴细胞亚群 白介素-5 白介素-25 哮喘控制测试 相关性 

分 类 号：R725.6[医药卫生—儿科]