桂枝汤标准汤剂及配方颗粒中芍药苷、肉桂酸和甘草酸铵的含量对比研究  

Comparative study on the content of Peony glycoside,Cinnamic acid and Ammonium glycyrrhizinate in Guizhi Decoction Standard Decoction and Formula Granule Decoction

作　　者：罗玉梅[1] 黄新刚[1] 邹天志 王超[1] 邹芙蓉[3] LUO Yu-mei;HUANG Xin-gang;ZOU Tian-zhi(Sheng Li Oil-field Central Hospital,Dongying 257034,China;不详)

机构地区：[1]胜利油田中心医院,山东东营257034 [2]乳山市卫生健康事务服务中心,山东乳山264500 [3]威海市立医院,山东威海264200

出　　处：《中国处方药》2023年第2期38-42,共5页Journal of China Prescription Drug

基　　金：2019年全国中药特色技术传承人才培训项目(国中医药人教函[2019]43号,T20194828003)。

摘　　要：目的采用高效液相色谱-二极管阵列检测法(HPLC-DAD)测定桂枝汤标准汤剂与桂枝汤配方颗粒中芍药苷、肉桂酸和甘草酸铵三种成分的含量并进行对比。方法采用JADE-PAK KPEC-C_(18)(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,柱温35℃,流速1.0 ml/min。结果芍药苷、肉桂酸和甘草酸铵分别在2.57~51.44μg/ml、2.00~40.19μg/ml、2.66~53.26μg/ml浓度范围内有良好的线性关系(r2值分别为0.9998、0.9997和0.9999),检测限(RSN=3)分别为0.086μg/ml、0.033μg/ml和0.177μg/ml;精密度RSD为0.65%、0.33%和0.41%;平均回收率为96.2%、98.6%和96.4%。结论桂枝汤标准汤剂中的芍药苷与肉桂酸含量高于配方颗粒,而甘草酸铵的含量低于配方颗粒。本研究能为中药煎煮工艺提供技术参考,为规范配方颗粒临床用药和统一配方颗粒质量提供理论依据。Objective A high performance liquid chromatography with diode array detection(HPLC-DAD)method was developed for the simultaneous determination of three compounds including paeonia sinensis,cinnamic acid and ammonium glycyrrhizinate and for comparing the content of above components of Guizhi Standard Decoction and Guizhi Formula Granules Decoction.Methods The samples were separated with an ODS-C_(18) column(250 mm×4.6 mm,5μm)by gradient elution using water containing 0.1%phosphoric acid and acetonitrile as mobile phase,with a flow rate of 1 ml/min at 35℃.Results The standard curves revealed a good linear relationship(r2=0.9998,0.9997 and 0.9999)in the ranges of 2.57~51.44μg/ml for paeonia sinensis;2.00~40.19μg/ml for cinnamic acid and 2.66~53.26μg/ml for ammonium glycyrrhizinate,respectively.The limits of detection were 0.086,0.033,and 0.177μg/ml(RSN=3),respectively.The RSD of precision were 0.65%,0.33%and 0.41%,respectively.The average recovery rates were 96.2%,98.6%and 96.4%,respectively.Conclusion The contents of peony glycoside and cinnamic acid in Guizhi Decoction are shown to be higher than those in Guizhi Formula Granules Decoction.This study provides a technical reference for the decoction of traditional Chinese medicine,and provides a theoretical basis for standardizing the clinical use of formula granules and the quality of unified formula granules.

关 键 词：桂枝标准汤剂 配方颗粒 芍药苷 肉桂酸 甘草酸铵 含量测定 

分 类 号：R286.0[医药卫生—中药学]