重复经颅磁刺激对后肢去负荷小鼠神经元兴奋性及离子通道影响的研究  

Effects of repetitive transcranial magnetic stimulation on neuronal excitability and ion channels in hindlimb unloading mice

作　　者：侯文涛 付蕊 朱明强 朱海军 丁冲 HOU Wentao;FU Rui;ZHU Mingqiang;ZHU Haijun;DING Chong(Hebei Key Laboratory of Bioelectromagnetics and Neural Engineering,School of Health Sciences&Biomedical Engineering,Hebei University of Technology,Tianjin 300130,P.R.China;Tianjin Key Laboratory of Bioelectricity and Intelligent Health,School of Health Sciences&Biomedical Engineering,Hebei University of Technology,Tianjin 300130,P.R.China;Key Laboratory of Digital Medical Engineering of Hebei Province,College of Electronic and Information Engineering,Hebei University,Baoding,Hebei 071002,P.R.China)

机构地区：[1]河北工业大学、生命科学与健康工程学院、河北省生物电磁与神经工程重点实验室,天津300130 [2]河北工业大学、生命科学与健康工程学院、天津市生物电工与智能健康重点实验室,天津300130 [3]河北大学、电子信息工程学院、河北省数字医疗工程重点实验室,河北保定071002

出　　处：《生物医学工程学杂志》2023年第1期8-19,共12页Journal of Biomedical Engineering

基　　金：国家自然科学基金面上项目(52077057)。

摘　　要：本研究从神经电生理角度探究重复经颅磁刺激对模拟失重小鼠神经元兴奋性和离子通道的影响。将青年C57小鼠分成对照组、尾吊组和磁刺激组。对尾吊组和磁刺激组小鼠进行14天的尾吊处理,建立模拟失重模型,同时对磁刺激组小鼠施加14天的重复经颅磁刺激。利用离体脑片膜片钳实验,检测动作电位的相关指标和电压门控型钠、钾离子通道的动力学特性变化,分析神经元的兴奋性及其离子通道机制。结果显示,小鼠的行为认知能力和神经元兴奋性随着尾吊而显著下降,重复经颅磁刺激可以显著改善后肢去负荷小鼠海马齿状回颗粒细胞的认知损伤和神经电生理相关指标;重复经颅磁刺激可能是通过促进钠离子外流和抑制钾离子,来改变钠、钾离子通道的激活、失活和失活后再复活过程,影响离子通道的动力学特性,从而增强单个神经元的兴奋性,改善后肢去负荷小鼠的认知损伤和空间记忆能力。Weightlessness in the space environment affects astronauts’ learning memory and cognitive function. Repetitive transcranial magnetic stimulation has been shown to be effective in improving cognitive dysfunction. In this study, we investigated the effects of repetitive transcranial magnetic stimulation on neural excitability and ion channels in simulated weightlessness mice from a neurophysiological perspective. Young C57 mice were divided into control, hindlimb unloading and magnetic stimulation groups. The mice in the hindlimb unloading and magnetic stimulation groups were treated with hindlimb unloading for 14 days to establish a simulated weightlessness model, while the mice in the magnetic stimulation group were subjected to 14 days of repetitive transcranial magnetic stimulation. Using isolated brain slice patch clamp experiments, the relevant indexes of action potential and the kinetic property changes of voltage-gated sodium and potassium channels were detected to analyze the excitability of neurons and their ion channel mechanisms. The results showed that the behavioral cognitive ability and neuronal excitability of the mice decreased significantly with hindlimb unloading. Repetitive transcranial magnetic stimulation could significantly improve the cognitive impairment and neuroelectrophysiological indexes of the hindlimb unloading mice. Repetitive transcranial magnetic stimulation may change the activation, inactivation and reactivation process of sodium and potassium ion channels by promoting sodium ion outflow and inhibiting potassium ion, and affect the dynamic characteristics of ion channels, so as to enhance the excitability of single neurons and improve the cognitive damage and spatial memory ability of hindlimb unloading mice.

关 键 词：重复经颅磁刺激 后肢去负荷小鼠 神经元兴奋性 电压门控型钠离子通道 电压门控型钾离子通道 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]