植物FtsZ基因家族生物信息学分析  

Bioinformatics Analysis of FtsZ Gene Family in Plants

作　　者：黄娟[1] 张鹏 宋勇[1] 马秋香 Huang Juan;Zhang Peng;Song Yong;Ma Qiuxiang(Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding(Ministry of Education),Horticulture College of Hunan Agricultural University,Changsha,410128;National Key Laboratory of Plant Molecular Genetics,Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai,200032)

机构地区：[1]湖南农业大学园艺学院,园艺作物种质创新与新品种选育教育部工程研究中心,长沙410128 [2]中国科学院分子植物科学卓越创新中心,植物分子遗传国家重点实验室,上海200032

出　　处：《分子植物育种》2023年第4期1164-1174,共11页Molecular Plant Breeding

基　　金：国家重点研发计划项目(No.2018YFD1000500;No.2019YFD1001100);现代农业产业技术体系建设专项(No.CARS-11)共同资助。

摘　　要：FtsZ(Filamentous temperature-sensitive protein Z)蛋白是一种高度保守的细菌和真核生物微管蛋白的同系物,在许多植物和藻类物种中存在,是质体分裂复合体的基本组成部分,在植物生长发育中发挥重要作用。本研究在一种藻类植物(红藻)、一种苔藓植物(小立碗藓)、两种单子叶植物(水稻和高粱)、四种双子叶植物(木薯,拟南芥,蓖麻和毛果杨)中共鉴定出21个Fts Zs成员,分析了其蛋白理化性质、二级结构、三级结构,预测了保守碱基、FtsZs成员的亚细胞定位、蛋白磷酸化位点并且构建了系统发育进化树。结果显示,FtsZs的脂肪系数、不稳定系数和总平均亲水性等理化性质存在很大的差异。FtsZ蛋白家族均不具跨膜结构域,也不含有信号肽;蛋白的二级结构主要是无规则卷曲和延伸链;保守基序分析发现,植物FtsZ家族基因在进化上是相对保守的;FtsZs成员的亚细胞定位分析发现,Fts Zs分布在叶绿体和细胞质中。本研究可为FtsZ基因家族成员的生物学功能研究提供理论基础。FtsZ(Filamentous temperature-sensitive protein Z)protein is a highly conserved tubulin homologous analogue in bacteria and eukaryotes.And it exists in many plants and algae species,and is the basic component of plastid division proteins.21 FtsZ members were identified in different species including algae(Cyanidioschyzon merolae)and bryophytes(Physcomitrella patens),monocotyledons(Oryza sativa and Sorghum bicolor)and dicotyledous(Manihot esculenta Crantz,Arabidopsis thaliana,Ricinus communis and Medicago truncatula),the types were1,1,2 and 4,respectively.The proteins physical and chemical properties and secondary structure,tertiary structure were analyzed,subcellular localization conservative motifs,phosphorylation sites were predicted and the phylogenetic tree was constructed in this study.The results showed that the physical and chemical properties of FtsZ proteins were significantly different,such as aliphatic index(fat coefficient),instability index and grand average of hydropathicity.FtsZ proteins family had neither transmembrane domains nor signal peptides.The secondary structure of the proteins mainly consisted of irregular coils and extended chains.The conserved motifs analysis showed that the Fts Z family members were conservative during evolution.Subcellular localization analysis showed that FtsZ proteins were distributed in chloroplasts and cytoplasm.This study would provide a theoretical basis for the study of the biological functions of Fts Z gene family members.

关 键 词：FtsZ基因家族 质体分裂 进化分析 生物信息学 

分 类 号：Q943.2[生物学—植物学]