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作 者:王春萍[1] 李怡斐[2] 张世才[2] 杨小苗 吴红[1] 雷开荣[1] 黄任中[2] 黄启中[2] 林清[1] Wang Chunping;Li Yifei;Zhang Shicai;Yang Xiaomiao;Wu Hong;Lei Kairong;Huang Renzhong;Huang Qizhong;Lin Qing(Chongqing Key Laboratory of Adversity Agriculture,Biotechnology Research Institute,Chongqing Academy of Agricultural Sciences,Chongqing,401329;Vegetable and Flower Research Institute,Chongqing Academy of Agricultural Sciences,Chongqing,401329)
机构地区:[1]重庆市农业科学院生物技术研究所,逆境农业研究重庆市市级重点实验室,重庆401329 [2]重庆市农业科学院蔬菜花卉研究所,重庆401329
出 处:《分子植物育种》2023年第4期1330-1338,共9页Molecular Plant Breeding
基 金:国家现代农业产业技术体系建设专项(CARS-24-A-04);重庆市技术创新与应用发展专项面上项目(cstc-2020jscx-msxmX0054);重庆市技术创新与应用发展专项重点项目(cstc2019jscx-gksbX0123)共同资助。
摘 要:为了加强辣椒耐低氮种质资源的利用,本研究对从国内外收集的102份辣椒种质资源进行了苗期耐低氮性综合评价。结果显示,相对叶面积、相对地上部分干重、相对根干重、相对整株干重和相对根冠比的变幅分别为0.40~1.71、0.48~1.93、0.58~2.00、0.50~1.92和0.86~2.16,变异系数均大于18%,说明基因型间耐低氮性差异较大。聚类分析将102份材料分为了4个类群,第Ⅰ类群的93份材料为中间型,第Ⅱ类群的6份材料为低氮敏感型,第Ⅲ、Ⅳ类群的3份材料为耐低氮型,耐低氮材料与其余材料间欧氏距离较大,有利于杂种优势组合的组配。本研究利用聚类分析和模糊隶属函数综合分析法筛选出的低氮敏感材料和耐低氮材料完全一致,证明了筛选结果的准确性,研究结果为辣椒耐低氮育种提供了理论依据。In order to strengthen the utilization of low-nitrogen tolerant pepper germplasms, the comprehensive evaluation of low-nitrogen tolerance of 102 pepper germplasm resources from home and abroad was carried out at seedling stage. It is showed that, the amplitude of the relative leaf area, relative dry weight of shoot, relative dry weight of plant and relative root-shoot ratio of dry weight was 0.4~1.71, 0.48~1.93, 0.58~2.00, 0.50~1.92 and 0.86~2.16,respectively, and the coefficients of variation of these indexes were more than 18%, indicating great difference of the low-nitrogen tolerance among genotypes. 102 pepper varieties were categorized into the following 4 groups,group I was medium nitrogen-tolerant type including 93 varieties, group II was low-nitrogen sensitive type including 6 varieties, and group III IV were low-nitrogen tolerant types including 3 varieties. The euclidean distance between the low-nitrogen tolerant materials and the other materials is large, which is beneficial to combination of heterosis combinations. The selected low-nitrogen sensitive materials and the low-nitrogen tolerant materials are completely consistent by using both the cluster analysis and the fuzzy membership function, which proved the accuracy of the screening results. This study provided a theoretical basis for low-nitrogen tolerance pepper breeding.
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