菟丝子总黄酮中拟雌激素作用的活性成分筛选  

Screening of active ingredients for estrogenic effect of total flavonoids from Cuscutae Semen

作　　者：岳霞 宋辉[1] 徐媛 刘迪施 孙向明 李文兰[1] YUE Xia;SONG Hui;XU Yuan;LIU Dishi;SUN Xiangming;LI Wenlan(School of Pharmacy,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药学院,哈尔滨150076

出　　处：《中国药房》2023年第5期569-574,共6页China Pharmacy

基　　金：黑龙江省重点研发计划项目(No.2022ZX02C08);大学生创新创业训练计划项目(No.202210240009)。

摘　　要：目的筛选菟丝子总黄酮中拟雌激素作用的活性成分。方法以小鼠子宫系数和子宫内膜厚度为评价指标,评估10批菟丝子总黄酮的拟雌激素作用,并建立其指纹图谱,标定共有峰;采用双变量相关性分析和灰色关联度分析对共有峰和上述两种指标的谱效关系进行分析,筛选拟雌激素作用的活性成分,并采用超高效液相色谱串联四极杆飞行时间质谱(UPLC-Q-TOF-MS)技术对活性成分进行表征。结果10批菟丝子总黄酮的拟雌激素作用均较好。菟丝子总黄酮正、负离子模式下分别得到28、33个共有峰,其中正离子模式下的7、10、12~16、26号峰及负离子模式下2、5、8、9、12、16、19、22~26号峰所代表的成分与菟丝子总黄酮拟雌激素作用高度相关。进一步鉴定发现,菟丝子总黄酮拟雌激素作用的活性成分分别为5,7,3′,4′-四甲氧基黄酮、6-O-(反式)对香豆酰基-呋喃果糖-(2→1)-吡喃葡萄糖苷、芦丁、山柰酚-3,7-双葡萄糖苷、芹菜素-7-O-葡萄糖苷、金丝桃苷、紫云英苷、槲皮苷、槲皮素、芹菜素、山柰酚、异鼠李素、杜鹃黄素、异槲皮苷、山柰酚-3-呋喃阿拉伯糖苷、2,6-十八碳二炔酸。结论本研究从菟丝子总黄酮中筛选出16种拟雌激素作用的化学成分,可为植物雌激素类药物的开发提供参考。OBJECTIVE To screen the active ingredient with estrogenic effect from total flavonoids of Cuscutae Semen.METHODS The estrogenic effect of total flavonoids from 10 batches of Cuscutae Semen was evaluated with mouse uterus coefficient and endometrial thickness as evaluation indexes,establish its fingerprint and calibrate the common peak.Common peak and spectrum-effect relationship of the above two indicators were analyzed by bivariate relationship analysis and grey correlation analysis to screen active components with estrogenic effect.UPLC-Q-TOF-MS technology was used to characterize the active components.RESULTS The estrogenic effect of total flavonoids from 10 batches of Cuscutae Semen was good.Twenty-eight and thirty-three common peaks of total flavonoids in Cuscutae Semen were obtained in the positive and negative ion modes respectively.The constituents represented by peaks 7,10,12-16,26 in positive ion mode and peaks 2,5,8,9,12,16,19,22-26 in negative ion mode were highly correlated with the estrogenic effect of total flavonoids from Cuscutae Semen.Further identification showed that the active substances with estrogenic effect from the total flavonoids of Cuscutae Semen were 5,7,3′,4′-tetramethoxyflavone,6-O-(trans)p-coumarin-furanfructose-(2→1)-glucopyranoside,rutin,kaempferol-3,7-diglucoside,apigenin-7-O-glucoside,hyperoside,baicalin,quercitin,quercetin,apigenin,kaempferol,isorhamnetin,rhododendron,isoquercetin,kaempferol-3-furan arabinoside,2,6-octadecanediacetylic acid.CONCLUSIONS A total of 16 chemical components with estrogenic effect are screened from total flavonoids of Cuscutae Semen in the study,which can provide reference for the development of phytoestrogens.

关 键 词：菟丝子总黄酮 活性成分 拟雌激素作用 谱效关系 

分 类 号：R917[医药卫生—药物分析学] S481.9[医药卫生—药学]