不同菌草基质栽培灵芝多指标质量评价  

Multifactor Quality Evaluation of Ganoderma lucidum Cultivated using Different JUNCAO Substrates

作　　者：林冬梅[1] 罗虹建[1] 林树钱[1] 王赛贞[1] 林占熺[1] 王联福 林辉[1] LIN Dongmei;LUO Hongjian;LIN Shuqian;WANG Saizhen;LIN Zhanxi;WANG Lianfu;LIN Hui(National Engineering Research Center of JUNCAO Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福建福州350002

出　　处：《福建农业学报》2022年第12期1579-1585,共7页Fujian Journal of Agricultural Sciences

基　　金：中央引导地方科技发展专项(2021L3008);福建省科技重大专项(2021NZ0101);福建农林大学学科交叉融合推动菌草科学及产业高质量发展项目(XKJC71202103A)。

摘　　要：【目的】以水溶性浸出物、灵芝多糖肽、灵芝酸、灵芝甾醇、单糖组成和分子量特征图谱为指标综合评价不同菌草基质栽培灵芝的品质。【方法】采用HPLC法测定多糖肽、灵芝酸、甾醇含量和单糖组成,运用统计学方法比较不同基质栽培灵芝的差异及其相关性。【结果】不同栽培基质的水溶性浸出物含量为14.21%~17.35%,灵芝多糖肽含量为3.80%~6.63%,灵芝酸总量为0.24%~0.53%,甾醇含量为31.43~69.16μg·g^(-1);主成分分析筛选出2个主成分,累计方差贡献率84.63%;相关性分析显示浸出物与活性成分呈正相关;单糖组成主要是葡萄糖和少量甘露糖;分子量特征图谱标定了6个共有峰,组间相似度大于0.98。【结论】不同基质栽培灵芝的活性物质含量存在显著差异,其中灵芝多糖肽和灵芝酸可作为灵芝质量评价重要指标;类芦、芦竹和五节芒栽培灵芝富含活性成分,这为科学利用菌草栽培灵芝提供参考。【Objective】To comprehensively evaluate the quality of Ganoderma lucidum(G.lucidum)cultivated in different JUNCAO substrates by using water-soluble extract,polysaccharide peptides,ganoderic acids,sterols,monosaccharides and molecular weight map as indicators.【Method】Contents of polysaccharide peptides,ganoderic acids,sterols,and monosaccharides in the water-soluble extract of G.lucidum cultivated in different substrates using JUNCAO as ingredients were determined by HPLC.Differences and correlations among the evaluation criteria were statistically analyzed,and a molecular weight map of the active substances constructed.【Result】The content of water-soluble extract of various G.lucidum samples ranged 14.21%–17.35%,that of polysaccharide peptides 3.80%–6.63%,that of total ganoderic acids0.24%–0.53%,and that of sterols 31.43–69.16μg·g^(-1).The contents of water extract and the active substances were positively correlated.Among these substances,two principal components contributed 84.63%cumulative variance.The monosaccharides in G.lucidum were mainly glucose,aside from a small amount of mannose.There were 6 common peaks in the molecular weight fingerprint that showed a similarity between groups greater than 0.98.【Conclusion】There were significant differences in the content of active substances in G.lucidum cultivated with different substrates,and polysaccharide peptide and ganoderic acids could be used as important indicators for quality evaluation.G lucidum cultivated with Neyraudia reynaudiana,Arundo donax and Miscanthus floridulus is rich in active ingredients,which provides reference for the scientific cultivation of G.lucidum of JUNCAO for.

关 键 词：菌草基质 质量评价 灵芝多糖肽 灵芝酸 甾醇 单糖组成 分子量特征图谱 

分 类 号：R284[医药卫生—中药学]