范可尼贫血互补群E基本特性及抗原表位的生物信息学分析  

Bioinformatics Analysis of Fundamental Properties and Antigenic Epitopes of FANCE

作　　者：王道[1] 符淳[1] WANG Dao;FU Chun(Department of Obstetrics and Gynecology,the Second Xiangya Hospital,Central South University,Changsha 410011,China)

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011

出　　处：《激光生物学报》2023年第1期76-88,共13页Acta Laser Biology Sinica

基　　金：国家自然科学基金面上项目(82271674);湖南省自然科学基金面上项目(2022JJ30804)。

摘　　要：范可尼贫血互补群E(FANCE)属于FA家族的重要成员之一,参与DNA链间交联损伤修复并在某些肿瘤中起重要作用。本研究运用生物信息学方法对FANCE的同源性、理化性质、亲/疏水性、信号肽、亚细胞定位、跨膜结构、蛋白结构、蛋白质互相作用、B/T细胞优势抗原表位以及肿瘤相关性进行预测。FANCE为无信号肽和跨膜区的疏水性蛋白,主要分布在细胞核和细胞质,具有较高的保守性。其二级结构以α-螺旋和无规则卷曲为主,含有2个N-糖基化、9个O-糖基化以及48个磷酸化位点,与FANCM、FANCD2、FANCC等蛋白发生相互作用。FANCE具有多个潜在的B/T细胞优势抗原表位和17个抗原决定簇,在急性髓性白血病(LAML)和皮肤黑色瘤(SKCM)中低表达,其低表达显著影响患者总生存率。这为深入研究FANCE在肿瘤中的分子机制提供理论依据,使FANCE可能成为新的治疗靶点。Fanconi anemia complementation group protein E(FANCE), which is a member of FA family, plays a critical role in DNA interstrand crosslink(ICL) repair and contributes to progression of tumor. We used series of bioinformatics to predict and analyze the properties of human FANCE, involving homology, physicochemical property, hydrophobicity, signal peptide,subcellular localization, protein structure, protein-protein interaction, B/T cell epitopes and its correlation with tumor. FANCE was hydrophobic protein, without signal peptide and transmembrane region, mainly distributed in nucleus and cytoplasm. It showed being high conserved in evolution, of which secondary structure was characterized as α-helices and random coils, and had 2 N-glycosylation, 9 O-glycosylation and 48 phosphorylation sites. FANCE also could interact with FANCM, FANCD2,FANCC etc., and had many potential B/T cell epitopes and 17 antigenic determinants. FANCE was lowly expressed in LAML and SKCM, low FANCE expression reduced overall patient survival in SKCM. This study provides a theoretical basis for further researching the molecular mechanism of FANCE in tumors, which may offer potential as a novel therapeutic target.

关 键 词：FANCE 生物信息学 结构 抗原表位 肿瘤 

分 类 号：Q987[生物学—遗传学] R318.04[生物学—人类学]