Generation of oxidants and their role in the metabolism of oxygen in the survivability of filarial parasite Setaria digitata in cattle:a possible intervention strategy  

作　　者：Kochukunju Pillai Jayakumar Kottayath G Nevin 

机构地区：[1]Department of Biochemistry,PMS College of Dental Science and Research,Thiruvananthapuram 695028,India [2]Centre for Bioactive Substances from Marine Organisms,Kerala University of Fisheries and Ocean Studies,Kerala 682506,India [3]Department of Marine Biosciences,Faculty of Ocean Science and Technology,Kerala University of Fisheries and Ocean Studies,Kerala 682506,India

出　　处：《Infectious Diseases Research》2023年第1期23-27,共5页传染病研究（英文）

摘　　要：Background:The goal of the current study was to identify the potential therapeutic targets by analyzing the production of oxidants and their effects on the antioxidant system of Setaria digitata,a filarial worm that was collected from the peritoneal cavity of recently butchered calves.Methods:S.digitata was collected and the mitochondria-like particles(MLP),somatic isolates and the post-nuclear fraction were prepared for investigation.The radical intensity and the antioxidant enzyme activity in these fractions were evaluated.The effect of diethylcarbamazine(DEC),Q0,omeprazole,and L-methyl arginine on NOS production in the worm was also evaluated.Results:Experiments showed that coelomic fluid and whole worm showed significant levels of Nitric oxide compared to other fractions.The cytosol of the worm showed higher NOS activity than MLP.Superoxide dismutase and nitric oxide(NO)levels were found to be higher in MLP fraction of the worm.It was also found that DEC did not have any effect on the prevention of NO,while Q0,omeprazole,and L-methyl arginine treatment showed a decrease in NO production significantly.Conclusion:The results showed that by using the existing drugs we could reduce the propagation of S.digitata by inhibiting the production of NO.

关 键 词：Setaria digitata mitochondria-like particles nitric oxide L-methyl arginine 

分 类 号：F42[经济管理—产业经济]