基于LC-MS的蛇倒退抗肿瘤活性部位筛选及鉴定研究  

Research on Isolation and Identification of Antitumor Active Components from Shedaotui (Polygonum perfoliatum L.) Based on LC-MS

作　　者：姚琛 洪梦莹 林心怡 方圆 赵少萍 屈珈羽 杨晓兰 郑晓亮 陶锋[1] YAO Chen;HONG Mengying;LIN Xinyi;FANG Yuan;ZHAO Shaoping;QU Jiayu;YANG Xiaolan;ZHENG Xiaoliang;TAO Feng(Hangzhou Medical College,Hangzhou,Zhejiang 310053)

机构地区：[1]杭州医学院,浙江杭州310053

出　　处：《中国中医药科技》2023年第2期232-235,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：杭州医学院2021年国家级创新训练项目(202113023008)。

摘　　要：目的:分离得到蛇倒退中抗肿瘤活性部位并鉴定。方法:通过硅胶柱色谱分离蛇倒退得到不同馏分,以SGC-7901的抑制率和IC50为指标筛选得到抗肿瘤作用最强部位,并在倒置显微镜下观察细胞形态及应用LC-MS对其进行鉴定。结果:经分离得到4个主要馏分,其中馏分1的抗肿瘤活性最强,LC-MS鉴定得到一系列化合物。结论:蛇倒退中分离得到的PEC A部位具有良好的抗肿瘤活性,其主要成分为黄酮类化合物。Objective: To isolate and identify the antitumor components from Polygonum perfoliatum L. Methods: Different fractions were separated by silica gel column chromatography. The strongest anti-tumor fraction was screened by the inhibition rate and IC50of SGC-7901, and identified by LC-MS. Results: Four fractions were isolated, of which fraction 1 had the strongest antitumor activity. Then a series of compounds were identified by LC-MS.Conclusion: The PEC A fraction isolated from Polygonum perfoliatum L. has good anti-tumor activity, and its main components are flavonoids.

关 键 词：蛇倒退 PEC A 抗肿瘤 LC-MS 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]