^(12)C^(6+)辐射黄瓜M1代诱变效应研究  

Study on mutagenic effect of ^(12)C^(6+) radiation on M1 Generation of Cucumber

作　　者：蔡世娟 苏晓静 秦垒 曹天光[1] 耿金鹏 CAI Shijuan;SU Xiaojing;QIN Lei;CAO Tianguang(School of Science,Hebei University of Technology,Tianjin 300401 China)

机构地区：[1]河北工业大学理学院,天津300401 [2]不详

出　　处：《河北工业大学学报》2023年第1期13-19,65,共8页Journal of Hebei University of Technology

基　　金：河北省高等学校科学技术研究项目(ZD2017023)。

摘　　要：利用^(12)C^(6+)重离子对自交系黄瓜种子(18C-1)进行60 Gy、80 Gy、100 Gy、120 Gy剂量辐射,通过对种子发芽势、发芽率、发芽指数、成苗率、植株性状和功能、果实产量和品质以及SSR分子标记分析,探究重离子辐射黄瓜M1代种子生物学效应。结果表明,从辐射种子活力和成苗率上分析,辐射对种子活力和成苗率有显著地抑制作用,且随辐射剂量增加呈现先降后升变化趋势;从植株性状和功能上分析,高剂量辐射(100 Gy和120 Gy)植株受损严重不利于后代有效变异筛选;从株型的差异显著性和单株聚类图上分析,辐射对幼苗株型影响效应显著,且辐射剂量越接近影响效应越相似;从果实产量和品质上分析,60 Gy组筛选到果实性状优良、品质高的正向突变株;从SSR分子标记上分析,重离子辐射能诱发黄瓜基因组变异,其中120 Gy组尤为突出,60 Gy次之。综上所述,^(12)C^(6+)辐射对黄瓜M1代生物学性状和基因组DNA都产生显著影响,确定60 Gy为最适诱变剂量并筛选出正向突变株。本研究将为后续黄瓜诱变育种研究以及种质资源创新奠定基础。Using^(12)C^(6+)heavy ion to radiate the seeds of inbred Cucumber(18C-1) in 60 Gy, 80 Gy, 100 Gy and 120 Gy doses, the biological effects of heavy ion radiation on M1 Generation of Cucumber were studied by analyzing the seed germination potential, germination rate, germination index, seedling rate, plant character and function, fruit yield and quality and SSR molecular markers. The results showed that, from the perspective of radiation seed vigor and seedling rate, radiation significantly inhibited the seed vigor and seedling rate, and showed a trend of decreasing first and then increasing with the increase of radiation dose;from the analysis of plant character and function, high dose radiation(100 Gy and120 Gy) seriously damaged the plants, which was not conducive to the effective variation screening of offspring;from the analysis of plant type difference significance and single plant cluster diagram, the results showed that the effect of radiation on the plant type of seedlings was significant, and the closer the radiation dose was, the much more similar the effect was;from the analysis of fruit yield and quality, the positive mutants with good fruit characters and high quality were screened from the 60 Gy group;from the SSR molecular marker analysis, the heavy ion radiation could induce Cucumber genome variation, especially in the 120 Gy group, followed by the 60 Gy group. In conclusion,^(12)C^(6+)radiation had a significant impact on the biological characteristics and genomic DNA of M1 Generation of Cucumber. 60 Gy was determined as the optimal mutagenic dose and the positive mutant was screened out. This study will lay a foundation for the further research of Cucumber mutation breeding and germplasm resource innovation.

关 键 词：黄瓜 重离子辐射 种子萌发指标 田间性状 SSR分子标记 

分 类 号：Q947.9[生物学—植物学]