基于流式细胞术和K-mer方法测定6种槭属植物基因组大小  被引量:1

Estimation of genome sizes of six Acer species by flow cytometry and K-mer analysis

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作  者:马秋月 王玉虓 李倩中[1] 李淑顺[1] 闻婧[1] 朱璐 颜坤元 杜一鸣 解志军 李淑娴 欧阳芳群 鲁成代 MA Qiuyue;WANG Yuxiao;LI Qianzhong;LI Shushun;WEN Jing;ZHU Lu;YAN Kunyuan;DU Yiming;XIE Zhijun;LI Shuxian;OUYANG Fangqun;LU Chengdai(Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;CoInnovation Center for Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037,China;Forestry Science and Technology Spreading Station in Xiangyang,Xiangyang 441000,China;Beijing Botanical Garden,Beijing 100093,China;Institute of Acer pentaphyllum Plant Protection in Tianshui,Tianshui 741000,China)

机构地区:[1]江苏省农业科学院休闲农业研究所,江苏南京210014 [2]南京林业大学林学院,南方现代林业协同创新中心,江苏南京210037 [3]襄阳市林业科学技术推广站,湖北襄阳441000 [4]北京市植物园,北京100093 [5]天水市五小叶植物保护研究所,甘肃天水741000

出  处:《南京林业大学学报(自然科学版)》2023年第1期163-170,共8页Journal of Nanjing Forestry University:Natural Sciences Edition

基  金:国家自然科学基金项目(32001357);江苏省自然科学基金项目(BK20211139);国家重点研发计划(2022YFD2200401)。

摘  要:【目的】槭属植物种类繁多,具有观赏、油用、药用等重要价值,开发应用前景广阔。基于流式细胞术和K-mer分析方法,首次进行6种槭树基因组大小测定,为槭属植物分子生物学、细胞学研究提供重要理论参考。【方法】以6种槭树嫩叶为材料,WPB为细胞核解离液,大豆(Glycine max‘Polanka’)和玉米(Zea maysCE-777)为内标,利用流式细胞检测技术和K-mer分析法估测6种槭树的基因组大小。【结果】基于流式细胞术测定建始槭(Acer henryi)、三角枫(A.buergerianum)、秀丽槭(A.elegantulum)、血皮槭(A.griseum)、五小叶槭(A.pentaphyllum)和青楷槭(A.tegmentosum)的基因组大小分别为(691.12±8.69)、(863.90±8.69)、(896.50±4.35)、(893.24±8.69)、(766.10±8.69)和(1 154.04±13.04) Mb;以K-mer分析法估测的基因组大小分别为561.72、743.00、777.87、771.51、650.64和1 103.46 Mb;杂合率分别为1.00%、1.24%、1.31%、1.08%、0.28%、0.81%。K-mer分析法估测的基因组大小略小于流式细胞术预测结果。【结论】本研究的结果可丰富槭属植物基因信息库,也可为该属植物基因组学、分子细胞遗传学等研究奠定重要基础。【Objective】There are variety species in the genus Acer, which have broad prospects for development and application in the ornamental, oil and medicinal fields. To provide an important theoretical reference for molecular biology and cytology studies of Acer, the genome sizes of six species of Acer were estimated using flow cytometry and K-mer analysis. 【Method】Fresh leaves of six species of Acer were collected, and woody plant buffer(WPB) was used as a nuclear dissociation solution, Glycine max ‘Polanka’ and Zea mays CE-777 as internal standard. Six genome sizes were estimated by flow cytometry and K-mer analysis. 【 Result】The results of flow cytometry showed that the genomes of Acer henryi, A. buergerianum, A. elegantulum, A. griseum, A. pentaphyllum and A. tegmentosum were successfully estimated. The genome sizes of those were(691.12±8.69),(863.90±8.69),(896.50±4.35),(893.24±8.69),(766.10±8.69) and(1 154.04±13.04) Mb, respectively. The K-mer analysis showed that they were 561.72, 743.00, 777.87, 771.51, 650.64 and 1 103.46 Mb, respectively. The hybridity rate was about 1.00%、1.24%、1.31%、1.08%、0.28% and 0.81%, respectively, which were slightly smaller than that flow cytometry. 【Conclusion】The results of this study could enrich the Acer gene database and provide important foundations for genomic and molecular cytogenetics studies within this genus.

关 键 词:槭属 基因组大小 流式细胞术 K-mer分析 

分 类 号:S687.9[农业科学—观赏园艺] S722[农业科学—园艺学]

 

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