基于高效液相指纹图谱及网络药理学预测分析山银花质量标志物  

Prediction and analysis of the quality marker of Lonicerae Flos by HPLC fingerprint and network pharmacology

作　　者：周新茹 王志辉[1,2] 龙雨青 曾梅 杨敏 童巧珍 周日宝[1,2,3] 刘湘丹 ZHOU Xin-ru;WANG Zhi-hui;LONG Yu-qing;ZENG Mei;YANG Min;TONG Qiao-zhen;ZHOU Ri-bao;LIU Xiang-dan(School of Pharmacy,Hunan University of Traditional Chinese Medicine;Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Bulk Genuine Medicinal Materials;Hunan Provincial Key Laboratory of Modernization of Traditional Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学药学院 [2]湘产大宗道地药材种质资源及规范化种植重点研究室 [3]湖南省普通高等学校中药现代化研究重点实验室,长沙410208

出　　处：《天然产物研究与开发》2023年第2期208-220,241,共14页Natural Product Research and Development

基　　金：湖南省自然科学基金(2021JJ30497,2021JJ30515);国家现代农业产业技术体系(CARS-21);湖南中医药大学研究生创新课题(2021CX80)。

摘　　要：山银花具清热解毒、疏散风热功效,应用前景广阔。目前《中国药典》仅将绿原酸、灰毡毛忍冬皂苷乙及川续断皂苷乙作为山银花质量评价指标,不能全面评价山银花质量。本研究建立了山银花HPLC指纹图谱,对29批山银花进行相似度评价,应用聚类分析和正交偏最小二乘法判别分析(OPLS-DA)初步筛选出山银花8个潜在Q-Marker;通过网络药理学构建“成分-靶点-通路”网络图,进一步分析验证山银花潜在Q-Marker的有效性。本研究发现绿原酸、异绿原酸A、新绿原酸、獐芽菜苷、异绿原酸B、灰毡毛忍冬皂苷乙、咖啡酸、川续断皂苷乙可作为山银花质量标志物,其为较全面评价山银花药材质量提供了科学依据。Lonicerae Flos has the function of clear away heat and toxic material and treat the exopathogenic wind-heat,which has a broad application prospect.Only chlorogenic acid,macranthoidin B and dipsacoside B were used as quality evaluation indexes of Lonicerae Flos in Chinese Pharmacopoeia at present,which can′t comprehensively evaluate the quality of Lonicerae Flos.In this study,we have establelished the HPLC fingerprints of Lonicerae Flos,and evaluated the similarity of 29 batches of Lonicerae Flos.We screened the potential Q-marker of Lonicera Flos by orthogonal partial least squares discriminant analysis(OPLS-DA).We analyze and verify the effectiveness of the potential Q-Marker of Lonicera Flos by constructing a"component-target-pathway"network diagram through network pharmacology.The research results showed that chlorogenic acid,isochlorogenic acid A,neochlorogenic acid,sweroside,isochlorogenic acid B,macranthoidin B,caffeic acid,and dipsacoside B can be used as quality markers of Lonicera Flos,which can provide scientific basis for the quality control and evaluation of Lonicera Flos.

关 键 词：山银花 HPLC指纹图谱 多元统计分析 网络药理学 质量标志物 

分 类 号：R284.1[医药卫生—中药学]