假蒟叶的生物碱类成分研究  

Alkaloids from leaves of Piper sarmentosum

作　　者：邵金华 刘依林 吕敬崑 陈小明 SHAO Jin-hua;LIU Yi-lin;LYU Jing-kun;CHEN Xiao-ming(School of Chemistry and Bioengineering,Hunan University of Science and Engineering;Hunan Provincial Engineering Research Center for Ginkgo Folium,Hunan University of Science and Engineering;Hunan Hengwei Pharmaceutical Co.,Ltd.,Yongzhou 425100,China)

机构地区：[1]湖南科技学院化学与生物工程学院 [2]湖南省银杏工程技术研究中心 [3]湖南恒伟药业股份有限公司,永州425100

出　　处：《天然产物研究与开发》2023年第2期231-235,共5页Natural Product Research and Development

基　　金：湖南省自然科学基金(2020JJ9026)。

摘　　要：研究假蒟(Piper sarmentosum)叶的化学成分及其对金黄色葡萄球菌的抑菌活性。采用正相硅胶柱、Sephadex LH-20凝胶柱和半制备高效液相色谱等分离技术,从假蒟叶的总生物碱部位分离得到7个生物碱类成分,结合化合物的理化性质和波谱学数据,分别鉴定为chaplupyrrolidone C(1)、chaplupyrrolidone A(2)、chaplupyrrolidone B(3)、假蒟酰胺A(4)、N-3-苯丙酰胺吡咯(5)、3-(4-羟基-3,5-二甲氧基苯基)丙酰胺吡咯(6)、3-(3,4,5-三甲氧基苯基)丙酰胺吡咯(7)。其中,化合物1为新的酰胺类生物碱,化合物7为首次从假蒟中分离得到。采用微量二倍稀释法测试所有分离化合物的抑菌活性,结果表明化合物1~4对金黄色葡萄球菌均具有不同程度的抑制作用。The chemical composition of Piper sarmentosum leaves and their antibacterial activity against Staphylococcus aureus were investigated.Seven alkaloid components were isolated and purified from the total alkaloid parts of P.sarmentosum leaves by repeated column chromatography such as silica gel,Sephadex LH-20 and semi-preparative HPLC.According to the spectroscopic data,combined with the physical and chemical properties,their structures were identified as chaplupyrrolidone C(1),chaplupyrrolidone A(2),chaplupyrrolidone B(3),sarmentamide A(4),N-(3-phenylpropanoyl)pyrrole(5),3-(4-hydroxy-3,5-dimethoxyphenyl)propanoylpyrrole(6),3-(3,4,5-trimethoxyphenyl)propanoylpyrrole(7).Accordingly,compound 1 is discovered to be a new amide alkaloid and compound 7 was isolated for the first time from P.sarmentosum.The microbial inhibition activity of all the isolated compounds was tested by the broth microdilution method,and the results showed that compounds 1-4 exhibited different degrees of inhibition against Staphylococcus aureus.

关 键 词：假蒟 酰胺类生物碱 化学成分 金黄色葡萄球菌 

分 类 号：R284.1[医药卫生—中药学]