盘龙七片中抗炎镇痛生物碱类成分同时测定的方法学研究  

Study on the method of simultaneous determination of anti-inflammatory and analgesic alkaloids in Panlongqi tablets

作　　者：何姬 刘妍如[1] 段金廒[2] 唐志书[1,3] 宋忠兴 赵艳婷[1] 张德柱 杨国伟 HE Ji;LIU Yan-ru;DUAN Jin-ao;TANG Zhi-shu;SONG Zhong-xing;ZHAO Yan-ting;ZHANG De-zhu;YANG Guo-wei(Medicine Shaanxi Provincial and Ministerial Collaborative Innovation Center for Industrialization of Traditional Chinese Medicine Resources,Shaanxi University of Chinese Medicine,Xianyang 712083,China;Jiangsu Key Laboratory for High Technology Research of TCM Formulae,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing 210023,China;China Academy of Chinese Medical Sciences,Beijing 100700,China;Shaanxi Panlong Pharmaceutical Group Co.,Ltd.,Shangluo 711400,China;Shanxi Provincial Inspection and Testing Center(Shanxi Institute of Standards and Metrology Technology),Taiyuan 030002,China)

机构地区：[1]陕西中医药大学,陕西中药资源产业化省部共建协同创新中心,咸阳712083 [2]南京中医药大学,江苏省中药资源产业化过程协同创新中心,江苏省方剂高技术研究重点实验室,南京210023 [3]中国中医科学院,北京100700 [4]陕西盘龙药业集团股份有限公司,商洛711400 [5]山西省检验检测中心(山西省标准计量技术研究院),太原030002

出　　处：《天然产物研究与开发》2023年第2期291-301,352,共12页Natural Product Research and Development

基　　金：陕西省科技厅重点产业创新链(群)项目(2020ZDLSF05-08);国家自然科学基金面上项目(82173970);陕西省高层次人才特殊支持计划&青年拔尖人才支持计划(2018-33)。

摘　　要：采用超高效液相色谱-四极杆-飞行时间质谱联用(UPLC-Q-TOF-MS)技术,对盘龙七片抗炎镇痛生物碱类成分进行表征,共表征出17种生物碱成分;根据表征的结果建立UPLC-ESI-MS/MS定量分析方法以用于盘龙七片质量分析评价,结果表明17种生物碱在各自浓度范围内线性关系良好,r≥0.9990,仪器精密度、方法重复性、供试品稳定性均良好,平均加样回收率为86.42%~112.75%,RSD为1.49%~5.59%(n=6)。本研究所建立的方法准确、重复性良好,可用于盘龙七片中17种生物碱的定量测定,为完善盘龙七片质量控制的潜在新指标提供了参考。The ultra performance liquid chromatography tandem time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)technology was utilized to identify the anti-inflammatory and analgesic alkaloids components of Panlongqi tablets,and a total of seventeen alkaloids components were characterized;based on the characterization results,UPLC-ESI-MS/MS quantitative analysis method was established for the quality analysis of Panlongqi tablets.The results showed that seventeen alkaloids had good linear relationship in their respective concentration ranges,r≥0.9990,the average recoveries rate was 86.42%-112.75%,the RSDs of 1.49%-5.59%(n=6).The method established in this study is accurate and reproducible for the quantitative determination of seventeen alkaloids in Panlongqi tablets,which provides a reference for the improvement of potential new indicators for quality control of Panlongqi tablets.

关 键 词：盘龙七片 生物碱 成分分析 UPLC-Q-TOF-MS/MS UPLC-ESI-MS/MS 

分 类 号：R286.0[医药卫生—中药学] R917[医药卫生—中医学]