疏肝和胃方水提醇沉法的急性毒性试验及部分药效学研究  

Acute toxicity test of the water-extraction and alcohol-precipitation method for Shugan Hewei prescription and a partial pharmacodynamics study

作　　者：徐亭亭[1] 朱生樑[1] 周秉舵[1] 王轶[1] 王阿会 迪力库马尔·马坎 李亚鼎 XU Tingting;ZHU Shengliang;ZHOU Bingduo;WANG Yi;WANG Ahui;DILIKUMAER Makan;LI Yading(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《湖南中医杂志》2023年第1期168-172,共5页Hunan Journal of Traditional Chinese Medicine

基　　金：上海市2018年度科技创新行动计划生物医药领域科技支持项目(18401932600);上海市科学技术委员会科技创新行动计划项目(19401971800);上海中医药大学杏林传承型人才培养计划项目(上中医人字[2019]10号)。

摘　　要：目的:探索疏肝和胃方水提醇沉法的安全性并验证其疗效,为疏肝和胃方新药的推广提供实验依据。方法:1)急性毒性试验。将昆明小鼠分为药物高、中高、中、低剂量组及对照组,每组各10只,雌雄各半。灌胃剂量根据昆明小鼠10倍生药量计算,并按85%等比递减,按照4 ml/10 g体质量,1 d内灌胃2次,连续14 d。观察14 d内实验小鼠的一般情况、体质量变化、内脏湿重与内脏病理改变。2)药效学研究。将SD大鼠分为假手术组(0.9%氯化钠注射液1.5 ml)、模型组、中药组(中药混悬液1.5 ml)、西药组(奥美拉唑水溶液1.5 ml),每组各10只。对除假手术组外的其余组大鼠制备酸反流模型后给药,每天2次,连续14 d。观察各给药组大鼠食管病理改变及食管下段组织白细胞介素-1β(IL-1β)mRNA、白细胞介素-6(IL-6)mRNA表达情况。结果:急性毒性试验结果显示,各组小鼠精神状态良好,组间体质量差异无统计学意义(P>0.05),组间内脏湿重无明显改变、内脏无明显病理改变。药效学研究结果显示,中药组大鼠食管下段组织黏膜上皮基底层更为平坦,棘细胞减少,无黏膜糜烂与溃疡。各给药组大鼠食管IL-1βmRNA、IL-6 mRNA表达较模型组、西药组下降,差异有统计学意义(P<0.05)。结论:疏肝和胃方水提醇沉法安全性高,且可修复食管黏膜,抑制食管炎症。Objective:To investigate the safety of the water-extraction and alcohol-precipitation method for Shugan Hewei prescription and its clinical effect,and to provide an experimental basis for promoting the new drug of Shugan Hewei prescription.Methods:In the acute toxicity test,Kunming mice were divided into high-,medium-high-,middle-,and low-dose drug groups and control group,with 10 mice(5 male mice and 5 female mice)in each group.The dose for gavage was calculated as 10 times of crude drug in Kunming mice and was reduced proportionally at a percentage of 85%,and the drug was given by gavage twice a day at a dose of 4 ml/10 g body weight for 14 consecutive days.The mice were observed in terms of general status,change in body weight,visceral wet weight,and pathological changes of viscera.In the pharmacodynamic study,Sprague-Dawley rats were divided into sham-operation group(0.9%sodium chloride injection 1.5 ml),model group,traditional Chinese medicine(TCM)group(TCM suspension 1.5 ml),and Western medicine group(omeprazole solution 1.5 ml),with 10 rats in each group.All rats except those in the sham-operation group were used to establish a model of acid reflux,and then the drug was given twice a day for 14 consecutive days.The rats were observed in terms of the pathological changes of the esophagus and the mRNA expression levels of interleukin-1β(IL-1β)and interleukin-6(IL-6)in the lower segment of the esophagus.Results:The acute toxicity test showed that the mice in each group had a good general status,and there was no significant difference in body weight between groups(P>0.05),while there were no obvious changes in visceral wet weight or no pathological changes of viscera.The pharmacodynamic study showed that the TCM group had a smooth basal layer of the mucosal epithelium in the lower segment of the esophagus and a reduction in prickle cells,with no mucosal erosion or ulceration.The medication groups had significantly lower mRNA expression levels of IL-1βand IL-6 than the model group and the Western medicine gr

关 键 词：疏肝和胃方 提取方法 急性毒性试验 药效学实验 

分 类 号：R285.5[医药卫生—中药学]