一株新分离雨生红球藻培养条件的优化  

Optimization of culture conditions for a newly isolated microalga Haematococcus pluvialis

作　　者：戴颖 穆亮亮 黄旭雄[1,2,3] DAI Ying;MU Liangliang;HUANG Xuxiong(Key Laboratory of Freshwater Aquatic Germplasm Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Shanghai Aquaculture Engineering Technology Research Center,Shanghai Ocean University,Shanghai 201306,China;National Experimental Teaching Demonstration Center of Fishery Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学农业农村部淡水水产种质资源重点实验室,上海201306 [2]上海海洋大学上海市水产养殖工程技术研究中心,上海201306 [3]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《上海海洋大学学报》2023年第1期68-78,共11页Journal of Shanghai Ocean University

基　　金：上海市科技兴农项目(沪农科推字〔2019〕第2-5号)。

摘　　要：为探究一株新分离雨生红球藻的适宜生长条件,以藻细胞密度为响应值,通过单因子试验方法,获得该株雨生红球藻生长的最佳光照强度、培养温度和接种密度分别为105μmol/(m^(2)·s)、21℃和7.5×10^(4)cells/mL。然后采用响应曲面法(RSM)耦合Box-Behnken设计,建立了预测雨生红球藻细胞密度的二次多项式方程,培养光照强度、温度和接种密度及其二项式对雨生红球藻细胞培养密度有着显著影响。各因素影响强弱的顺序:接种密度>光照强度>培养温度。响应曲面法获得雨生红球藻的最佳培养条件为光照强度120μmol/(m^(2)·s),温度20℃,接种密度9.25×10^(4)cells/mL,在此组合条件下雨生红球藻细胞密度可达45.08×10^(4)cells/mL,比单因素实验得到的最高藻细胞密度高出6.9%。The suitable culture conditions for the growth of a newly isolated microalga Haematococcus pluvialis were investigated.The light intensity,culture temperature and inoculation density for optimal growth of the microalga were obtained as 105μmol/(m^(2)·s),21℃and 7.5×10^(4)cells/mL respectively based on cell density as the indicator via single factor test.The growth performance(cell density)of the microalga could be predicted with the quadratic polynomial equation established by response surface method(RSM)coupled with Box-Behnken design.Light intensity,culture temperature and inoculation density as well as their binomial had significant effects on the cell density of H.pluvialis in cultivation.The weight of influence from the factors was as follows:inoculation density>light intensity>culture temperature.The combined culture conditions for optimal growth of H.pluvialis predicted by RSM were light intensity 120μmol/(m^(2)·s),temperature 20℃and inoculation density 9.25×10^(4)cells/mL.Confirmatory test showed a cell density of 45.08×10^(4)cells/mL in culture was obtained under the predicted optimal combined culture conditions,which was 6.9%higher than the highest cell density of the culture under optimal culture conditions based on single factor test.

关 键 词：雨生红球藻 生长 响应曲面法 光照强度 培养温度 接种密度 

分 类 号：S963.21[农业科学—水产养殖] Q94-33[农业科学—水产科学]