青田田鱼幼鱼脑组织在急性低氧胁迫、复氧恢复的转录组分析  

Transcriptome analysis of brain tissue of juvenile Qingtian Paddy Field Carp(Cyprinus carpio var.qingtianensis)during acute changes in dissolved oxygen

作　　者：刘韬 齐明 高阳[1,2,3] 刘其根[1,2,3] LIU Tao;QI Ming;GAO Yang;LIU Qigen(National Experimental Teaching Demonstration Center of Fisheries Science,Shanghai Ocean University,Shanghai 201306,China;Research Center for Fish Nutrition and Environmental Ecology,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Shanghai Aquaculture Engineering Technology Research Center,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]上海海洋大学农业农村部鱼类营养与环境生态研究中心,上海201306 [3]上海海洋大学上海市水产养殖工程技术研究中心,上海201306

出　　处：《上海海洋大学学报》2023年第1期79-88,共10页Journal of Shanghai Ocean University

基　　金：上海高校知识服务平台资助项目(ZF1206)。

摘　　要：为了解青田田鱼(Cyprinus carpio var.qingtianensis,Paddy Field Carp)对稻田急性溶氧变化的水环境适应性,依据青田田鱼对低氧耐受性参数及当地稻田水环境溶氧日变化的实际情况模拟急性低氧和复氧环境,基于RNA-seq技术对青田田鱼幼鱼常氧对照组[温度:(25.37±0.76)℃,溶氧:(7.07±0.39)mg/L,CB组],低氧胁迫6 h组[温度:(25.65±0.76)℃,溶氧:(0.57±0.16)mg/L,HB组]和复氧恢复6 h组[温度:(25.78±0.58)℃,溶氧:(7.21±0.53)mg/L,RB组]脑组织进行转录组分析。结果表明:HB组与CB组文库比较发现存在1094个差异基因,其中,730个上调,364个下调;RB组与HB组文库比较发现2288个差异基因,其中,803个上调,1485个下调;RB组与CB组文库比较发现201个差异基因,其中,80个上调,121个下调。进一步将差异表达基因进行GO功能注释和KEGG富集分析发现差异基因主要富集在糖酵解/糖异生、HIF-1信号、mTOR信号及VEGF信号等通路上。通过RT-qPCR对糖酵解/糖异生及相关通路中的6个差异基因进行验证,证实了转录组测序结果的可靠性。这些结果为研究青田田鱼在急性溶氧变化环境下的生理调节机制提供了分子生物学的基础研究。In order to explore the adaptability of Cyprinus carpio var.qingtianensis(Paddy Field Carp)to acute dissolved oxygen changes in paddy fields,Our treatment conditions simulated the acute hypoxia and reoxygenation environment according to the tolerance parameters of Cyprinus carpio var.qingtianensis and the daily situation in the local paddy field water environment,and transcriptome analysis was conducted in brain tissue samples from normal oxygen control group(CB groups),hypoxia stress 6 h group(HB groups)and reoxygenation group(RB groups)by RNA-seq technology.The results showed that there were 1094 differential genes in the library of the HB groups and the CB groups,of which 730 were up-regulated and 364 were down-regulated;the comparison of the RB groups and the HB groups library revealed 2288 differential genes,of which 803 were up-regulated and 1485 were down-regulated;the RB groups library revealed 201 differential genes,of which 80 were up-regulated and 121 were down-regulated compared with CB groups.Further,GO and KEGG analysis indicated that acute oxygen changes were involved in multiple biological pathways,mainly glycolysis/gluconeogenesis,HIF-1 signaling pathway,mTOR signaling pathway,VEGF signaling pathway and insulin signaling pathway.Finally,6 differential genes were verified in the glycolysis/gluconeogenesis and related pathways by RT-qPCR experiments,which confirmed the reliability of the transcriptome sequencing results.These results provided basic molecular biology information for studying the physiological regulation mechanism of Cyprinus carpio var qingtianensis in the environment of acute changes in dissolved oxygen.

关 键 词：青田田鱼 低氧胁迫 复氧恢复 转录组 

分 类 号：S917.4[农业科学—水产科学]