绵羊日粮中添加亚硒酸钠对胎盘中SelP和ApoER-2蛋白表达的影响  

Effect of Sodium Selenite Supplementation in Sheep Diets on Expression of SelP and ApoER-2 Proteins in the Placenta

作　　者：刘卓 牛宏泽 裴世腾 任有蛇[1] 石磊[1] LIU Zhuo;NIU Hongze;PEI Shiteng;REN Youshe;SHI Lei(College of Animal Science,Shanxi Agricultural University,Laboratory for Animal Reproductive Biotechnology,Shanxi Taigu 030801,China)

机构地区：[1]山西农业大学动物科学学院,动物繁殖生物技术实验室,山西太谷030801

出　　处：《饲料工业》2023年第4期55-60,共6页Feed Industry

基　　金：山西省普通高等学校大学生创新创业训练项目[20210142];山西省现代农业羊产业技术体系项目。

摘　　要：试验通过在妊娠母羊日粮中添加不同水平的亚硒酸钠,研究硒对绵羊胎盘SelP和ApoER-2的蛋白表达的影响,为进一步揭示硒的胎盘转运及硒对胎儿发育的调控机制提供实验依据。选取体重、体型、年龄、胎次相近的湖羊妊娠母羊80只,随机分为两组,分别饲喂基础日粮和添加0.5 mg/(kg DM·d)亚硒酸钠的日粮。预试期7 d,正试期150 d。结果表明:SelP和ApoER-2主要在妊娠母羊胎盘滋养层细胞中表达。日粮中亚硒酸钠的添加,极显著提高了妊娠母羊胎盘滋养层细胞中SelP的表达量(P<0.01),却极显著降低了ApoER-2蛋白的表达量(P<0.01)。The aim of this experiment is to investigate the effect of selenium on the protein expression of SelP and ApoER-2 in sheep placenta by adding different levels of sodium selenite to the diets of pregnant ewes, and to provide an experimental basis for further revealing the placental transport of selenium and the regulatory mechanism of selenium on fetal development. Eighty pregnant ewes of lake sheep with similar body weight, size, age and fecundity were randomly divided into two groups, and fed the basal diet and the diet supplemented with 0.5 mg/(kg DM·d)sodium selenite, respectively. It is concluded that SelP and ApoER-2 were mainly expressed in the placental trophoblast cells of pregnant ewes. The addition of sodium selenite to the diet increased the expression of SelP and decreased the expression of ApoER-2 protein in the placental trophoblast cells of pregnant ewes significantly(P<0.01).

关 键 词：湖羊 胎盘 硒蛋白P ApoER-2 基因表达 蛋白定位 

分 类 号：S816.32[农业科学—饲料科学]