麻黄-细辛药对提取物对哮喘模型小鼠气道重塑的影响  

Effect of herb pair extracts of Ephedra herba-Asari radix et rhizoma on airway remodeling in asthmatic model mice

作　　者：陈宏[1] 张明浩 陈群[2] 宋倩男 张佩颖 赵建云 隋博文[3] CHEN Hong;ZHANG Minghao;CHEN Qun;SONG Qiannan;ZHANG Peiying;ZHAO Jianyun;SUI Bowen(The First Department of Pediatrics,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Department of Laboratory Medicine,Harbin Acheng District People's Hospital,Harbin 150300,Heilongjiang,China;Department of Respiratory,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学附属第一医院儿一科,黑龙江省哈尔滨市150040 [2]黑龙江省哈尔滨市阿城区人民医院检验科,黑龙江省哈尔滨市150300 [3]黑龙江中医药大学附属第一医院呼吸科,黑龙江省哈尔滨市150040

出　　处：《广西医学》2023年第2期162-167,共6页Guangxi Medical Journal

基　　金：黑龙江省自然科学基金(H2018052);中国博士后科学基金特别资助项目(2020T130178)。

摘　　要：目的探讨麻黄-细辛药对提取物对哮喘小鼠模型气道重塑的影响。方法将30只Balb/c小鼠分为对照组、模型组、麻黄-细辛组,各10只。除对照组外,其他两组建立哮喘小鼠模型。建模后第18天起给予麻黄-细辛组麻黄-细辛药对提取物雾化吸入治疗,给予对照组和模型组小鼠等体积生理盐水雾化吸入。观察各组小鼠肺组织形态,检测各组小鼠增强呼气间歇值(Penh),肺支气管肺泡灌洗液(BALF)中各类白细胞比例,BALF和血清的白细胞介素(IL)-6和IL-17水平,以及肺组织的Yes相关蛋白(YAP)、YAP mRNA、转化生长因子β1(TGF-β1)蛋白、α-平滑肌肌动蛋白(α-SMA)的表达水平。结果各组小鼠吸入相应浓度乙酰甲胆碱后,模型组小鼠Penh均高于对照组,麻黄-细辛组小鼠Penh均低于模型组(均P<0.05)。模型组小鼠的BALF中嗜酸性粒细胞、淋巴细胞、单核细胞及中性粒细胞比例,BALF和血清中IL-6及IL-17水平,以及肺组织中TGF-β1蛋白和α-SMA表达水平,YAP及其mRNA表达水平均高于对照组(均P<0.05);与模型组比较,麻黄-细辛组上述指标水平均降低(均P<0.05)。结论麻黄-细辛药对提取物能够减轻哮喘模型小鼠的气道反应性和炎症反应,并可能通过下调IL-17、IL-6、TGF-β1、α-SMA、YAP等因子的表达而抑制哮喘模型小鼠的气道重塑。Objective To explore the effect of herb pair extracts of Ephedra herba-Asari radix et rhizoma on airway remodeling in asthmatic model mice.Methods A total of 30 Balb/c mice were divided into control group,model group,or Ephedra herba-Asari radix et rhizoma group,with 10 mice in each group.Except for the control group,asthmatic mice model was constructed in the remaining two groups.On the 18thday after modeling,the Ephedra herba-Asari radix et rhizoma group received aerosol inhalation of Ephedra herba-Asari radix et rhizoma herb pair extracts for treatment,and the control and model groups received aerosol inhalation of normal saline with equivalent volume.The pulmonary tissue morphology of mice in various groups was observed,and the enhanced pause(Penh),proportions of various leucocytes in pulmonary bronchoalveolar lavage fluid(BALF),and levels of interleukin(IL)-6 and IL-7 in BALF and serum,as well as expressions of Yes associated protein(YAP),YAP mRNA,transforming growth factorβ1(TGF-β1)protein,andα-smooth muscle actin(α-SMA)in pulmonary tissues of mice were detected in various groups.Results After methacholine with corresponding concentration was inhaled in mice of various groups,the model group exhibited a higher Penh as compared with the control group,and the Ephedra herba-Asari radix et rhizoma group depicted a lower Penh as compared with the model group(all P<0.05).The model group yielded higher proportions of eosinophils,lymphocytes,monocytes,and neutrophils in BALF,and higher levels of IL-6 and IL-17 in BALF and serum,as well as higher expressions of TGF-β1 protein,α-SMA,YAP,and YAP mRNA in pulmonary tissues as compared with the control group(all P<0.05).Compared with the model group,the Ephedra herba-Asari radix et rhizoma group interpreted decreased levels of aforesaid indices(all P<0.05).Conclusion Ephedra herba-Asari radix et rhizoma herb pair extracts can relieve airway reactions and inflammatory reactions of asthmatic model mice,and inhibit airway remodeling of asthmatic model mice by down-reg

关 键 词：哮喘 麻黄-细辛 提取物 气道重塑 炎症反应 小鼠 

分 类 号：R562.25[医药卫生—呼吸系统] R282.6[医药卫生—内科学]