丁酸梭菌对肉鸡生长、肠道形态及屏障功能的影响  

Effects of Clostridium butyricum on Growth,Intestinal Morphology and Barrier Function of Broilers

作　　者：李文嘉 徐彬[1] 魏凤仙[1] 邓文[1] 马慧慧[1] 王琳燚[1] 白杰[1] 孙全友[1] 席燕燕 李绍钰[1] LI Wenjia;XU Bin;WEI Fengxian;DENG Wen;MA Huihui;WANG Linyi;BAI Jie;SUN Quanyou;XI Yanyan;LI Shaoyu(Institute of Animal Husbandry and Veterinary Science,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院畜牧兽医研究所,河南郑州450002

出　　处：《河南农业科学》2023年第1期144-153,共10页Journal of Henan Agricultural Sciences

基　　金：国家现代农业产业技术体系资助项目(CARS‐41);河南省农业科学院优秀青年科技基金项目(2020YQ27);河南省农业科学院自主创新项目(2023ZC054);河南省高层次人才国际化培养项目。

摘　　要：为研究日粮中添加丁酸梭菌(Clostridium butyricum)对肉鸡生长性能、肠道形态以及肠道屏障功能的影响,选取健康且规格一致的1日龄爱拔益加公仔鸡324只,随机分为3个处理,每个处理6个重复,每个重复18只。对照组(CON)饲喂基础日粮,抗生素组(ANT)和丁酸梭菌组(CB)分别饲喂含10 mg/kg维吉尼亚霉素和1×10^(9)cfu/kg丁酸梭菌的日粮。试验周期为42 d。分别于21、42日龄,以重复为单位统计分析各组的生长性能和肠道组织形态,以及肠道黏膜细胞焦亡和肠道屏障相关基因mRNA表达丰度等指标。结果表明,与对照组相比,抗生素组和丁酸梭菌组的平均日增质量(ADG)以及末质量(FBW)分别显著升高6.31%、8.43%和6.26%、8.31%。42日龄,丁酸梭菌组半胱天冬酶-1(Caspase‐1)、白介素-18(IL‐18)和白介素-1β(IL‐1β)水平较对照组分别显著下降24.91%、42.96%和19.91%。与对照组相比,丁酸梭菌组空肠绒毛高与绒毛高/隐窝深(V/C)比值分别显著提高13.40%和54.20%;回肠隐窝深度显著降低32.62%,V/C比值显著升高60.76%。42日龄,与对照组相比,丁酸梭菌和抗生素组空肠黏膜IL-1β、肿瘤坏死因子-α(TNF-α)、Caspase-1和IL-18 mRNA相对表达水平显著降低,且丁酸梭菌组闭合小环蛋白-1(ZO-1)和闭合蛋白-1(Claudin-1)mRNA表达水平均显著上调。综上,日粮中添加1×10^(9)cfu/kg丁酸梭菌能提高肉鸡的生长性能和饲料利用率,改善肉鸡肠道形态,提升肠道免疫和屏障功能,并能在一定程度上缓解肉鸡的肠道细胞焦亡。The experiment was conducted to investigate the effects of Clostridium butyricum in diet on growth performance,intestinal morphology and intestinal barrier function of broilers.A total of 324 healthy one‐day‐old Arbor Acres male broilers with the same specifications were randomly divided into 3 treatments with 6 replicates per treatment and 18 broilers per replicate.Control group(CON)was fed with a basal diet,antibiotic group(ANT)and Clostridium butyricum group(CB)were fed with the basal diet containing 10 mg/kg virginiamycin and 1×109 cfu/kg Clostridium butyricum,respectively.The experiment period was 42 days.At the age of 21 and 42 days,the growth performance,intestinal tissue morphology,intestinal mucosal pyroptosis and intestinal barrier related gene mRNA expression abundance of each group were statistically analyzed.The results showed that compared with the CON group,the average daily gain(ADG)and final body weight(FBW)in ANT group and CB group were significantly increased by 6.31%and 8.43%,and 6.26%and 8.31%,respectively.At 42 days,the levels of Caspase‐1,IL‐18 and IL‐1βin CB group were significantly decreased by 24.91%,42.96%and 19.91%compared with the CON group,respectively.The jejunum villus height and villus height/crypt depth(V/C)ratio were significantly increased by 13.40%and 54.20%,respectively;and the ileum crypt depth was significantly decreased by 32.62%,and the V/C ratio of ileum was significantly increased by 60.76%.At 42 days,the mRNA relative expression levels of IL‑1β,TNF‑α,Caspase‑1 and IL‑18 in jejunum mucosa in CB and ANT groups were significantly lower than those in CON group and the expression levels of ZO‑1 and Claudin‑1 in CB group were significantly up‐regulated,respectively.In conclusion,dietary supplementation of 1×109 cfu/kg C.butyricum can improve the growth performance and feed utilization of broilers,improve the intestinal morphology,enhance the intestinal immune and barrier functions,and alleviate the intestinal cell pyroptosis of broilers to a cert

关 键 词：肉鸡 丁酸梭菌 生长 肠道形态 肠道屏障功能 

分 类 号：S816[农业科学—饲料科学]