Metabolomics-centered mining of plant metabolic diversity and function:Past decade and future perspectives  

作　　者：Shuangqian Shen Chuansong Zhan Chenkun Yang Alisdair R.Fernie Jie Luo 

机构地区：[1]Sanya Nanfan Research Institute of Hainan University,Hainan Yazhou Bay Seed Laboratory,Sanya 572025,China [2]College of Tropical Crops,Hainan University,Haikou 570228,China [3]Max Planck Institute of Molecular Plant Physiology,Potsdam-Golm 14476,Germany

出　　处：《Molecular Plant》2023年第1期43-63,共21页分子植物（英文版）

基　　金：Hainan Yazhou Bay Seed Lab(no.B21HJ0903);"111"Project(no.D20024);National Natural Science Foundation of China(no.32100318);China National Postdoctoral Program for Innovative Talents(BX20220097);China Postdoctoral Sci-ence Foundation(2022M710991 and,2021TQ0093);Hainan Yazhou Bay Seed Laboratory(B21Y10904);Hainan University Startup Fund(KYQD(ZR)1866);Hainan Provincial Natural Science Foundation of China(321QN184).

摘　　要：Plants are natural experts in organic synthesis,being able to generate large numbers of specific metabolites with widely varying structures that help them adapt to variable survival challenges.Metabolomics is a research discipline that integrates the capabilities of several types of research including analytical chemistry,statistics,and biochemistry.Its ongoing development provides strategies for gaining a systematic understanding of quantitative changes in the levels of metabolites.Metabolomics is usually performed by targeting either a specific cell,a specific tissue,or the entire organism.Considerable advances in science and technology over the last three decades have propelled us into the era of multi-omics,in which metabolomics,despite at an earlier developmental stage than genomics,transcriptomics,and proteomics,offers the distinct advantage of studying the cellular entities that have the greatest influence on end phenotype.Here,we summarize the state of the art of metabolite detection and identification,and illustrate these techniques with four case study applications:(i)comparing metabolite composition within and between species,(ii)assessing spatio-temporal metabolic changes during plant development,(iii)mining characteristic metabolites of plants in different ecological environments and upon exposure to various stresses,and(iv)assessing the performance of metabolomics as a means of functional gene identification,metabolic pathway elucidation,and metabolomics-assisted breeding through analyzing plant populations with diverse genetic variations.In addition,we highlight the prominent contributions of joint analyses of plant metabolomics and other omics datasets,including those from genomics,transcriptomics,proteomics,epigenomics,phenomics,microbiomes,and ion-omics studies.Finally,we discuss future directions and challenges exploiting metabolomics-centered approaches in understanding plant metabolic diversity.

关 键 词：metabolomics multi-omics HIGH-THROUGHPUT metabolic profiling bioactive markers 

分 类 号：Q75[生物学—分子生物学]