Genome-wide identification and comparative analysis of lipocalin families in Lepidoptera with an emphasis on Bombyx mori  

作　　者：Yan-Yan Zhou Yue Jin Shuai-Qi Liu Shi-Liang Xu Yu-Xin Huang Yu-Song Xu Lian-Gen Shi Hua-Bing Wang 

机构地区：[1]Department of Economic Zoology,College of Animal Sciences,Zhejiang University,Hangzhou,China

出　　处：《Insect Science》2023年第1期15-30,共16页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(grant no.31970460,32170483);the Natural Science Foundation of Zhejiang Province(grant no.LR22C040001);the Zhejiang Provincial Science and Technology Plans(2021C02072-6);the Zhejiang Provincial Key Laboratory Construction Plans(2020E10025).

摘　　要：Lipocalins exhibit functional diversity,including roles in retinol transport,invertebrate cryptic coloration,and stress response.However,genome-wide identification and characterization of lipocalin in the insect lineage have not been thoroughly explored.Here,we found that a lineage-specific expansion of the lipocalin genes in Lepidoptera occurred in large part due to tandem duplication events and several lipocalin genes involving insect coloration were expanded more via tandem duplication in butterflies.A comparative analysis of conserved motifs showed both conservation and divergence of lepidopteran lipocalin family protein structures during evolution.We observe dynamic changes in tissue expression preference of paralogs in Bombyx mori,suggesting differential contribution of paralogs to specific organ functions during evolution.Subcellular localization experiments revealed that lipocalins localize to the cytoplasm,nuclear membrane,or nucleus in BmN cells.Moreover,several lipocalin genes exhibited divergent responses to abiotic and biotic stresses,and 1 lipocalin gene was upregulated by 30o fold in B.mori.These results suggest that lipocalins act as signaling components in defense responses by mediating crosstalk between abiotic and biotic stress responses.This study deepens our understanding of the comprehensive characteristics of lipocalins in insects.

关 键 词：functional divergence gene duplication LEPIDOPTERA lipocalin 

分 类 号：Q96[生物学—昆虫学]