Functional map of the macroglomerular complex of male Helicoverpaarmigera  

作　　者：Xiao-Lan Liu Xi Chu Long-Long Sun Ya-Nan Wang Gui-Ying Xie Wen-Bo Chen Yang Liu Bente GBerg Shi-Heng An Gui-Rong Wang Xin-Ming Yin Xin-Cheng Zhao 

机构地区：[1]Henan International Joint Laboratory of Green Pest Control,College of Plant Protection,Henan Agricultural University,Zhengzhou,China [2]State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Bejing,China [3]Chemosensory Laboratory,Department of Psychology,Norwegian University of Science and Technology,Trondheim,Norway

出　　处：《Insect Science》2023年第1期109-124,共16页昆虫科学（英文版）

基　　金：supported by theNational Natural Science Foundation of China(U1604109,32130089,31861133019);the Program for Science and Technology Innovation Talents in Universities of Henan Province(19HASTITO11).

摘　　要：The mechanism of sex pheromone reception in the male cotton bollworm Helicoverpa armigera has been extensively studied because it has become an important model system for understanding insect olfaction.However,the pathways of pheromone processing from the antenna to the primary olfactory center in H.armigera have not yet been clarified.Here,the physiology and morphology of male H.armigera olfactory sensory neurons(OSNs)were studied using single sensillum recording along with anterograde filling and intracellular recording with retrograde filling.OSNs localized in type A sensilla responded to the major pheromone component cis-11-hexadecenal,and the axonal terminals projected to the cumulus(Cu)of the macroglomerular complex(MGC).The OSNs in type B sensilla respondcd to the bchavioral antagonist cis-9-tetradecenal,and the axonal terminals projected to the dorsomedial anterior(DMA)unit of the MGC.In type C sensilla,there were 2 OSNs:one that responded to cis-9-tetradecenal and cis-11-hexadecenol with the axonal terminals projecting to the DMA,and another that responded to the secondary pheromone components cis-9-hexadecenal and cis-9-tetradecenal with the axonal terminals projecting to the dorsomedial posterior(DMP)unit of the MGC.Type A and type B sensilla also housed the secondary OSNs,which were silent neurons with axonal terminals projected to the glomerulus G49 and DMP.Overall,the neural pathways that carry information on attractiveness and aversiveness in response to female pheromone components in H.armigera exhibit distinct projections to the MGC units.

关 键 词：intracellular recording/retrograde filling macroglomerular complex olfactory sensory neuron single sensillum recording/anterograde filling 

分 类 号：Q96[生物学—昆虫学]