Expression profile of CYP402C1 and its role in resistance to imidacloprid in the whitefly, Bemisia tabaci  

作　　者：Lei Guo Zhuang Zhang Wei Xu Jiangya Ma Ni Liang Changyou Li Dong Chu 

机构地区：[1]Key Lab of Integrated Crop Pest Management of Shandong Province,College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao,Shandong Province,China [2]Food Futures Institute,Murdoch University,Murdoch,WA,Australia

出　　处：《Insect Science》2023年第1期146-160,共15页昆虫科学（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(32072456 and 31601659);the Science and Technology Supporting Program for Young Innovative Teams in Higher Education of Shandong Province(2020KJF001);the Taishan Scholar Foundation of Shandong Province(tsqn20161040).

摘　　要：Bemisia tabaci(Gennadius)(Hemiptera:Aleyrodidae)is a cosmopolitan insect pest causing serious damage to crop production.Cytochromes P450(CYPs)of B.tabaci are widely known to be involved in the metabolic resistance to a variety of insecticides,continuously increasing the difficulty in controlling this pest.In this study,four P450 genes(CYP6CMI,CYP6CXl,CYP6CX3,and CYP402CI)in B.tabaci exhibited correlations with the resistance to imidacloprid.We have focused on trying to understand the function and metabolism capacity of CYP402C1.The expression profiles of CYP402C1 were examined by reverse transcription quantitative real-time PCR and fluorescence in situ hybridizations.Its role in resistance to imidacloprid was investigated by RNA interference,transgenic Drosophila melanogaster,and heterologous expression.The results showed that CYP402CI was highly expressed in the active feeding stages of B.tabaci,such as nymphs and female adults.CYP402C1 was mainly expressed in midguts of nymphs and adults,especially in the filter chamber.Knockdown of CYP402CI significantly decreased the resistance of B.tabaci to imidacloprid by 3.96-fold(50%lethal concentration:186.46 versus 47.08 mg/L).Overexpression of CYP402CI in a transgenic D.melanogaster line(Gal4>UAS-CYP402CI)significantly increased the resistance to imidacloprid from 12.68-to 14.92-fold(129.01 and 151.80 mg/L versus 1925.14 mg/L).The heterologous expression of CYP402CI showed a metabolism ability of imidacloprid(imidacloprid decreased by 12.51%within 2 h).This study provides new insights for CYP402C1 function in B.tabaci and will help develop new strategies in B.tabaci control and its insecticide resistance.

关 键 词：Bemisia tabaci gene expression NEONICOTINOID P450 RESISTANCE 

分 类 号：S48[农业科学—农药学]