基于产丁酸菌组合研究参苓白术散多糖体外降解的微生态机制  

Study on the microecological mechanism of polysaccharide degradation of Shenling Baizhu Powder in vitro based on butyric acid producing bacteria combination

作　　者：林萍 邱超 舒青龙[1] 封勇[1] LIN Ping;QIU Chao;SHU Qing-long;FENG Yong(College of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Air Force Hospital of Southern Theater Command of PLA,Guangzhou 510030,China)

机构地区：[1]江西中医药大学中医学院,南昌330004 [2]解放军南部战区空军医院,广州510030

出　　处：《中华中医药杂志》2023年第2期620-625,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82160788)。

摘　　要：目的:研究“拟杆菌/厚壁菌”与产丁酸菌组合对中药多糖体外降解机制。方法:以参苓白术散多糖为唯一碳源构建多糖培养基(PM),脑心浸液培养基(BHI)为对照,通过连续取样测定产丁酸菌在PM、及不同菌(包括脆弱拟杆菌、多形拟杆菌、厚壁菌YT2等)的PM降解液中的生长曲线,评估多糖体外降解机制。结果:产丁酸菌在多形拟杆菌降解的PM降解液中生长最好,在PM中的生长最差;PM/BHI分别经多糖降解菌降解后,PM中的产丁酸菌菌群数量更高;丁酸浓度变化中,经厚壁菌YT2的PM降解液最高,PM中产丁酸浓度最低;经多糖降解菌降解的PM降解液>经多糖降解菌降解的BHI降解液。结论:“拟杆菌/厚壁菌”产丁酸菌组合有利于对中药多糖的降解利用和丁酸生产。Objective: To study the degradation mechanism of traditional Chinese medicine polysaccharides in vitro by the combination of ’Bacteroides/firmicum’ and butyric acid producing bacteria. Polysaccharide medium(PM) was constructed with the polysaccharide of Shenling Baizhu Powder as the only carbon source,and brain heart infusion broth(BHI) was used as the control. Through continuous sampling,the growth curve of butyric acid producing bacteria in PM and PM degradation solution of different bacteria(including Bacteroides fragilis,Bacteroides thetaiotaomicron and Clostridium sp.YT2,etc.) was determined were measured to evaluate the degradation mechanism of polysaccharides in vitro. Results: Butyric acid producing bacteria grew best in PM degradation solution degraded by Bacteroides thetaiotaomicron and worst in PM. After PM/BHI was degraded by polysaccharide degrading bacteria,the number of butyric acid producing bacteria in PM was higher;Among the changes of butyric acid concentration,the PM degradation solution treated by thick walled Clostridium sp.YT2 was the highest,and the butyric acid concentration produced by PM was the lowest. PM degradation solution degraded by polysaccharide degrading bacteria > BHI degradation solution degraded by polysaccharide degrading bacteria. Conclusion: ’Bacteroides/firmicum’ combination of butyric acid producing bacteria is conducive to the degradation and utilization of traditional Chinese medicine polysaccharides and butyric acid production.

关 键 词：产丁酸菌 参苓白术散 多糖 碳源 多糖降解菌 

分 类 号：R285[医药卫生—中药学]