母婴分离产后抑郁大鼠脑内5-羟色胺及其代谢产物的变化及参芪解郁方的干预作用  

Changes of 5-hydroxytryptamine and metabolites in the brain of maternal and infant separation postpartum depressed rats and the intervention effects of Shenqi Jieyu Formula

作　　者：姜圣楠 曲淼 王丹 李净娅 杨歆科 田雷瑜 唐启盛 JIANG Sheng-nan;QU Miao;WANG Dan;LI Jing-ya;YANG Xin-ke;TIAN Lei-yu;TANG Qi-sheng(Beijing University of Chinese Medicine,Beijing 100029,China;Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Zhejiang Provincial Hospital of Chinese Medicine,Hangzhou 310006,China;Medical Experimental Teaching Center of School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学第三附属医院,北京100029 [3]浙江省中医院,杭州310006 [4]北京中医药大学中医学院医学实验教学中心,北京100029

出　　处：《中华中医药杂志》2023年第2期836-840,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81804024);中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者(No.国中医药人教发[2018]12号)。

摘　　要：目的:观察母婴分离产后抑郁大鼠前额叶皮质和海马5-羟色胺(5-HT)及其代谢产物5-羟吲哚乙酸(5-HIAA)的含量,探讨不同剂量参芪解郁方的干预作用。方法:60只SD孕鼠,随机分为正常组、模型组、中药高剂量组、中药中剂量组、中药低剂量组和西药组,采用母婴分离法制备产后抑郁大鼠,灌胃两周后检测各组大鼠前额叶皮质、海马5-HT及其代谢产物5-HIAA含量。结果:模型组强迫游泳不动时间较正常组显著增加(P<0.01),蔗糖水消耗量、旷场实验水平得分和垂直得分均较正常组显著降低(P<0.01)。中药高、中、低剂量组和西药组强迫游泳不动时间较模型组显著降低(P<0.01);中药高、中、低剂量组和西药组蔗糖水消耗量、旷场实验水平得分较模型组均显著升高(P<0.01,P<0.05);中药高、中剂量组和西药组旷场实验垂直得分较模型组均显著升高(P<0.01)。模型组前额叶皮质、海马5-HT及其代谢产物5-HIAA含量较正常组均显著降低(P<0.01)。中药高、中、低剂量组和西药组前额叶皮质、海马5-HT及其代谢产物5-HIAA含量较模型组均升高(P<0.01,P<0.05)。结论:参芪解郁方通过调节产后抑郁大鼠单胺类神经递质及其代谢产物水平发挥抗抑郁作用。Objective: To observe the contents of 5-hydroxytryptamine(5-HT) and its metabolite 5-hydroxyindolenacetic acid(5-HIAA) in prefrontal cortex and hippocampus of postpartum depression rats separated from mother and infant,and to explore the intervention effect of different doses of Shenqi Jieyu Formula. Methods: Sixty SD pregnant rats were randomly divided into normal group,model group,high dose group,medium dose group,low dose group and western medicine group. Postpartum depression rats were prepared by mother-infant separation method. After intragastric administration,the behavior of rats was evaluated and the contents of 5-HT and its metabolite 5-HIAA in prefrontal cortex and hippocampus of each group were detected.Results: The forced swim immobility time in the model group was significantly increased compared with that in the normal group(P<0.01),and the sucrose water consumption,the open field test level score,and the vertical score were all significantly decreased compared with the normal group(P<0.01). The immobility time of forced swimming in the Chinese medicine high-,medium-,and low-dose groups and the western medicine group was significantly decreased compared with that in the model group(P<0.01);sucrose water consumption and open field test level scores in the high-,medium-,and low-dose Chinese medicine groups and the western medicine group were increased compared with those in the model group(P<0.01,P<0.05). The vertical scores in the open field test in the high-,medium-dose groups,and western medicine group were significantly increased compared with those in the model group(P<0.01). The contents of 5-HT and its metabolite 5-HIAA in the prefrontal cortex and hippocampus in the model group were significantly lower than those in the normal group(P<0.01). The contents of 5-HT and its metabolite 5-HIAA in the prefrontal cortex and hippocampus in the high-,medium-and low-dose groups and the western medicine group were all higher than those in the model group(P<0.01,P<0.05). Conclusion: Shenqi Jieyu Formula e

关 键 词：母婴分离 产后抑郁 神经递质 5-羟色胺 5-羟吲哚乙酸 参芪解郁方 机制 

分 类 号：R285.5[医药卫生—中药学]