参竹心康方治疗慢性心力衰竭的作用机制  

To Explore the Mechanism of Shenzhu Xinkang Prescription in Treating Chronic Heart Failure based on Network Pharmacology

作　　者：赵梦雪 喻正科[2] 赵启[2] 李珊珊 陈静 ZHAO Mengxue;YU Zhengke;ZHAO Qi;LI Shanshan;CHEN Jing(Hunan University of Traditional Chinese Medicine,Hunan Changsha 410006,China;Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine,Hunan Changsha 410006,China)

机构地区：[1]湖南中医药大学,湖南长沙410006 [2]湖南省中医药研究院附属医院,湖南长沙410006

出　　处：《中医药临床杂志》2023年第2期265-272,共8页Clinical Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局第七批全国老中医药专家学术经验继承项目(国中医药人教函[2022]76号);湖南省科技厅科研计划项目(2020SK51001);湖南省中医药研究院科研计划联合重点项目(202118);湖南省中医药科研计划项目(202009)。

摘　　要：目的:基于网络药理学筛选参竹心康方主要活性化合物,预测参竹心康方在对于慢性心力衰竭治疗中的潜在作用靶点及信号通路,对其作用机制进行研究。方法:主要利用TCMSP平台并结合ETCM数据库检索参竹心康方各中药的化学成分及活性成分作用靶点;通过DisGeNET、DrugBank、OMIM、GeneCards数据库获取慢性心力衰竭的主要靶点;运用Cytoscape软件系统构建“药物-活性成分-靶点”网络及PPI网络,筛选核心靶点。采用Metascape数据平台对交集靶点进行GO功能富集分析和KEGG通路富集分析。结果:得到参竹心康方有效成分158个、药物靶点1688个、慢性心衰靶点1913个,最终获得参竹心康方作用于慢性心衰的关键靶点206个,KEGG通路204条。结论:参竹心康发治疗慢性心衰主要成分为槲皮素、山奈酚、木犀草素、黄芩苷、异鼠李素,关键靶点为AKT1、VEGFA、IL6、TNF、TP53,关键通路涉及PI3K-Akt信号通路、JAK-STAT信号通路、钙信号通路、cAMP信号通路、cGMP-PKG信号通路等。Objective:To screen the main active compounds of Shenzhu Xinkang Recipe based on network pharmacology,predict the potential targets and signaling pathways of Shenzhu Xinkang Recipe in the treatment of chronic heart failure,and study its mechanism of action.Methods:Mainly use the TCMSP platform combined with the ETCM database to retrieve the chemical components and active component targets of Shenzhu Xinkang prescription;obtain the main targets of chronic heart failure through DisGeNET,DrugBank,OMIM,and GeneCards databases;use Cytoscape software system Build a“drug-active ingredient-target”network and PPI network to screen core targets.The Metascape data platform was used to perform GO functional enrichment analysis and KEGG pathway enrichment analysis on the intersection targets.Results:158 active ingredients,1688 drug targets and 1913 chronic heart failure targets of Shenzhu Xinkang prescription were obtained,and finally 206 key targets and 204 KEGG pathways of Shenzhu Xinkang prescription acting on chronic heart failure were obtained.Conclusion:The main components of Shenzhu Xinkang in the treatment of chronic heart failure are quercetin,kaempferol,luteolin,baicalin,and isorhamnetin,and the key targets are AKT1,VEGFA,IL6,TNF,and TP53.The key pathways involve PI3K-Akt signaling pathway,JAK-STAT signaling pathway,calcium signaling pathway,cAMP signaling pathway,cGMP-PKG signaling pathway,etc.

关 键 词：参竹心康方 慢性心力衰竭 网络药理学 作用机制 

分 类 号：R541[医药卫生—心血管疾病]