HPLC-DAD/ELSD法测定当归-黄芪药对不同配比下主要有效成分的含量  

Active Ingredient of Angelicae Sinensis and Astragal membranaceus at Different Ratios by HPLC-DAD/ELSD Method

作　　者：李彦荣 牛淑秀 刘光炜 蒋宜伟 王小荣 LI Yanrong;NIU Shuxiu;LIU Guangwei;JIANG Yiwei;WANG Xiaorong(Dingxi Campus of Gansu University of Traditional Chinese Medicine,Dingxi 743000,China;Pharmaceutical of Dingxi People's Hospital,Dingxi 743000,China;Clinical College of Traditional Chinese Medicine of Cansu University of Traditional Chinese Medicinc,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学定西校区,定西743000 [2]定西市人民医院药剂科,定西743000 [3]甘肃中医药大学中医临床学院,兰州730000

出　　处：《中央民族大学学报（自然科学版）》2023年第1期79-85,共7页Journal of Minzu University of China(Natural Sciences Edition)

基　　金：国家自然科学基金(82160916);甘肃省科技计划项目(21JR1RA268):定西市重点技术攻关专项(DX2021AZ04)。

摘　　要：本文旨在建立高效、快速和精度高的方法测定当归-黄芪药对中主要有效成分阿魏酸、黄芪甲苷和毛蕊异黄酮葡萄糖苷含量,并探讨当归与黄芪按不同比例配伍时二者中主要有效成分的变化趋势。采用加热回流提取法制备当归、黄芪按5∶1、4∶1、3∶1、2∶1、1∶1、1∶2、1∶3、1∶4、1∶5比例配伍下的供试品溶液。选用SinoChromODS-AP(4.6 mm×250 mm,5μm)色谱柱,以乙腈和0.2%冰醋酸溶液为流动相梯度洗脱,体积流量为1.0 mL/min,柱温25℃,选择DAD检测器测定阿魏酸和毛蕊异黄酮葡萄糖苷,检测波长为260 nm和310 nm,黄芪甲苷的测定选择ELSD检测器。结果表明:所建立的检测方法线性关系良好,三种指标性成分加样回收率RSD%<3.0%,含量测定发现,黄芪甲苷、阿魏酸和毛蕊异黄酮葡萄糖苷含量随着当归、黄芪配比不同而发生变化。故HPLC-DAD/ELSD法检测操作快速、准确、简便、精密度高且重复性好,本研究初步揭示了当归-黄芪药对不同配比时的主要活性成分变化,为进一步探讨当归-黄芪配伍协同增效的药理学作用提供参考。To establish a method for simultaneous determination of ferulic acid,calycosin glycoside and astragaloside IV in Angelicae sinensis and Astragalus membranaceus,and to explore the change trend of the main active components in Angelica and Astragalus membranaceus when they were compatible in different proportions.Method:Angelica an∶Astragalus were prepared by heating and refluxing extraction in the proportions 5-1,4∶1,3∶1,2∶1,1∶1,1∶2,1∶3,1∶4,1∶5.Using SinoChromODS-AP(4.6 mm×250 mm,5μm)chromatographic column,gradient elution with acetonitrile and 0.2%glacial acetic acid solution as mobile phase,volume flow rate of 1.0 mL/min,column temperature of 25℃;The DAD detector was used for the detemination of ferulic acid and calycosin glycoside,and the detection wavelengths were 260 nm and 310 nm.The ELSD detector was selected for the determination of astragaloside IV.Results:The established detection method has a good linear relationship.The sample recovery rate of the three index components is RSD%<3.0%.The content determination shows that the content of ferulic acid,calycosin glycoside and astragaloside IV is the same as the ratio of angelica and astragalus.Conclusion:HPLC-DAD/ELSD method is simple and reproducible.

关 键 词：HPLC-DAD/ELSD法 当归-黄芪药对 阿魏酸 毛蕊异黄酮葡萄糖苷 黄芪甲苷 

分 类 号：R284.1[医药卫生—中药学]