基于UPLC-MS/MS技术的内源性胆汁酸分析方法研究进展  

Advances in analytical methods for endogenous bile acids based on UPLC-MS/MS technology

作　　者：魏娇娇 鄢星 梅余琪 丁丽丽[1] 李林楠 王峥涛[1,2] 杨莉 WEI Jiao-jiao;YAN Xing;MEI Yu-qi;DING Li-li;LI Lin-nan;WANG Zheng-tao;YANG Li(The MOE Key Laboratory of Standardization of Chinese Medicines,the SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines,Shanghai Key Laboratory of Compound Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;.Shanghai R&D Centerfor Standardization of Chinese Medicines,Shanghai 201203,China)

机构地区：[1]上海中医药大学中药研究所,中药标准化教育部重点实验室,国家中医药管理局中药新资源与质量评价重点实验室,上海市复方中药重点实验室,上海201203 [2]上海中药标准化研究中心,上海201203

出　　处：《药学学报》2023年第1期52-62,共11页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82274223,82074011);上海市晨光计划(20CG51);上海中医药大学“杏林百人”计划项目(20QN012)。

摘　　要：胆汁酸(bile acids,BAs)是一类调节脂质、葡萄糖和能量代谢的内源性甾体物质。其作为宿主和肠道微生物代谢的关键信号分子,在维持机体稳态和生理功能中发挥着重要的作用。生物体内BAs的准确定性定量在基础及临床研究中具有重要意义。过去几十年间,酶法分析、酶联免疫分析法、核磁共振、色谱法等相关技术均相继应用于BAs的检测。由于BAs结构多样、存在同分异构体,且生物样本基质复杂,给内源性BAs检测带来巨大挑战。超高效液相色谱-串联质谱(UPLC-MS/MS)是一种稳健性分析技术,其结合了UPLC快速的分离能力及MS/MS强大的结构鉴定功能,使得生物样品中目标分析物的快速分离、准确定性及定量检测更加便利。UPLC-MS/MS因具有高选择性、高灵敏度、高准确性等优势,近年来被广泛用于BAs的分析。本文主要对BAs的生物合成途径、样品前处理方法、常见分析检测技术进行总结,并重点介绍了近五年来UPLC-MS/MS技术在内源性BAs分析中的应用现状,旨在为内源性BAs准确检测及进一步研究和应用提供参考。Bile acids(BAs)are a group of endogenous steroid molecules that regulate lipid,glucose and energy metabolism.They play an important role in maintaining body homeostasis and physiological functions as key signaling molecules for host and gut microbial metabolism.The accurate characterization and quantification of BAs in vivo is of great importance in basic and clinical research.Over the past decades,enzymatic assay,enzymelinked immunoassay,nuclear magnetic resonance(NMR),chromatography,and other related techniques have been developed and applied to the detection of BAs.The diverse structures of BAs,the existence of isomers and the complex matrix of biological samples pose great challenges for the detection of endogenous BAs.Ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)is a robust analytical technique that combines the rapid separation capacities of UPLC with the powerful structural identification capabilities of MS/MS,facilitating the more rapid separation,characterization and accurate quantitative of target analytes in biological samples.UPLC-MS/MS has been widely used in the quantitative analysis of BAs in recent years for its high selectivity,high sensitivity,and high accuracy.This paper summarized the biosynthetic pathways of BAs,sample pretreatment methods,common analytical detection techniques,and highlights the current status of the application of UPLC-MS/MS technology in the analysis of endogenous BAs over the past five years,to provide a reference for the accurate detection of endogenous BAs and further research development and application.

关 键 词：胆汁酸 生物基质 样品前处理 液质联用 分析方法 

分 类 号：R917[医药卫生—药物分析学]