Qualitative and quantitative detection of aldehydes in DNA with 2-amino benzamidoxime derivative  

作　　者：Na Li Qian Zhou Kun Li Ting Jiang Xiao-Qi Yu 

机构地区：[1]Laboratory of Green Chemistry and Technology(Ministry of Education),College of Chemistry,Sichuan University,Chengdu 610064,China [2]Department of Chemistry,Xihua University,Chengdu 610039,China

出　　处：《Chinese Chemical Letters》2023年第1期317-321,共5页中国化学快报（英文版）

基　　金：financially supported by National Natural Science Foundation of China(Nos.22077088 and 21877082);Foundation from Science and Technology Department of Sichuan Province(Nos.2020JDJQ0017,2021YFH0132)。

摘　　要：An aldehyde-reactive probe based on 2-amino benzamidoxime(ABAO)framework was introduced,which can selectively label aldehydes in DNA through intramolecular ring closure under mild aqueous solutions.We screened ABAO derivatives that can undergo a cyclization with the formylated nucleobases to generate a fluorescence nucleoside,and of these derivatives 5–methoxy-ABAO(PMA)emerged as the optimal choice.PMA can sensitively and selectively react with 5f U,5f C and AP to form fluorogenic dihydroquinazoline derivatives,which also can quantify DNA damages induced byγ-irradiation.PMA-initiated labeling strategy provides great convenience for qualitative and quantitative detection of aldehydes in DNA.

关 键 词：Aldehydes in DNA 2-Amino benzamidoxime derivative Intramolecular ring closure Fluorescent adducts Quantitative analysis 

分 类 号：O657.3[理学—分析化学]