Study on ionic liquids based novel method for separation and purification of silkworm pupa protein  

作　　者：Wen Zhang Shaoqi Yang Baozeng Ren Xingmei Lu Cai Jia 

机构地区：[1]Beijing Key Laboratory of Ionic Liquids Clean Process,CAS Key Laboratory of Green Process and Engineering,State Key Laboratory of Multiphase Complex Systems,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China [2]Zhengzhou Institute of Emerging Industrial Technology,Zhengzhou 450000,China [3]Key Laboratory of New Materials and Facilities for Rural Renewable Energy of Ministry of Agriculture and Rural Affairs,College of Mechanical&Electrical Engineering,Henan Agricultural University,Zhengzhou 450002,China [4]School of Chemical Engineering,Zhengzhou University,Zhengzhou 450001,China

出　　处：《Chinese Chemical Letters》2023年第1期382-387,共6页中国化学快报（英文版）

基　　金：supported financially by the National Natural Science Foundation of China(Nos.81673400 and 21878292)。

摘　　要：Silkworm pupa protein(SPP)that obtained by traditional method usually had a high fat content,which would impose restrictions on the further use of SPP.Herein,various functionalized ionic liquids(ILs)were used to extract SPP from silkworm pupae,the structure-performance relationship of ILs with their SPP separation performance were explored at the same time.The research showed that the maximum extraction yield of SPP was up to 62.6%with less than 0.5%low fat content by using 1-ethyl-3-methylimidazolium chloride([Emim]Cl),when the dissolution experiment was conducted at 90°C for 24 h with ethanol bath as the regeneration solvent.Comparing with the structure of raw material,the regenerated SPP maintained the native protein backbone.Meanwhile,all regenerated SPP showed a decreased crystallinity,which also exhibited decreased fraction of theα-helix comparing to thatβ-sheet united with coil random structures.

关 键 词：Silkworm pupa protein Ionic liquids Protein backbone Silkworm pupa SEPARATION 

分 类 号：Q503[生物学—生物化学]