植物促生菌对设施土壤氮循环细菌群落及小白菜生长的影响  

Effects of plant growth promoting bacteria on soil nitrogen cycling bacterial community and growth of pakchoi in greenhouse

作　　者：陆文蔚 徐逍瑶 张丽雯 LU Wenwei;XU Xiaoyao;ZHANG Liwen(School of Hotel Management,Shanghai Business School,Shanghai 200235,China)

机构地区：[1]上海商学院酒店管理学院,上海200235

出　　处：《上海农业学报》2023年第1期1-8,共8页Acta Agriculturae Shanghai

基　　金：国家自然科学基金面上项目(31770088);上海商学院重点课程建设项目(AG-36602-0002)。

摘　　要：为研究植物促生菌S3-1菌株对设施土壤氮循环微生物数量、群落结构及小白菜生长的影响,比较了不同处理土壤中氨化细菌、亚硝化细菌、反硝化细菌及好氧性固氮菌的数量,并采用高通量测序分析amo A基因的多样性,同时对小白菜抗坏血酸、硝酸盐含量等指标进行测定。结果表明:菌悬液处理组(SBS)的小白菜叶片长度、可溶性糖和抗坏血酸含量比对照组(CK)分别提高23.14%、29.92%和32.04%,发酵液处理组(SFL)和菌体处理组(SC)小白菜的硝酸盐含量极显著低于LB处理组(P<0.01)。SBS组土壤的氨化细菌、亚硝化细菌、反硝化细菌与好氧性固氮菌的数量较CK和N处理组均有显著提高,其中亚硝化细菌和反硝化细菌数量增加更为显著,且两者之间的数量变化呈显著正相关。高通量测序结果显示:SFL处理组和SC处理组土壤中氨氧化细菌的丰富度指数均显著高于LB处理组;优势群落分析显示:SFL处理组丰度增加的主要是亚硝化螺菌属(Nitrosospira)细菌,而SC处理组丰度增加的主要是亚硝化单胞菌属(Nitrosomonas)细菌;独有种群分析显示:SFL处理组独有的OTUs数量显著高于SC处理组和LB处理组。以上研究表明:S3-1菌株对设施蔬菜营养物质的积累有促进作用,其菌体和发酵液短期内均可抑制蔬菜硝酸盐的积累,同时提高土壤中氮循环关键微生物的数量,对氨氧化细菌群落结构产生了显著影响,有利于提升设施土壤质量及蔬菜品质。In order to study the effects of plant growth promoting bacteria S3-1 strain on the number of nitrogen cycling microorganisms,community structure in greenhouse soil and the growth of pakchoi,the number of ammonifying bacteria,nitrobacteria,denitrifying bacteria and aerobic nitrogen-fixing bacteria in soil of different treatments were compared,and the diversity ofamoAgene was analyzed by high-throughput sequencing,and the ascorbic acid content,nitrate content and other indexes of pakchoi were measured. The results showed that the leaf length,soluble sugar content and ascorbic acid content of pakchoi in S3-1 bacterial suspension treatment(SBS) were 23. 14%,29. 92% and 32. 04% higher than those in the control( CK),respectively. The nitrate content of pakchoi in SFL treatment(S3-1 fermentation fertilizer) and SC treatment(S3-1 cells fertilizer) was significantly lower than that in LB treatment(P< 0. 01). The number of ammonifying bacteria,nitrobacteria,denitrifying bacteria and aerobic nitrogen-fixing bacteria in soil of SBS treatment were significantly higher than those of CK and N treatment, and the number of nitrobacteria and denitrifying bacteria increased more significantly,and there was a significant positive correlation between the number changes between them. The high-throughput sequencing results showed that the abundance index of ammonia-oxidizing bacteria in SFL treatment and SC treatment was significantly higher than that in LB treatment. The analysis of dominant communities showed that the abundance of SFL treatment increased mainly byNitrosospira,while the abundance of SC treatment increased mainly byNitrosomonas. The unique population analysis showed that the number of unique OTUs in SFL treatment was significantly higher than that in SC treatment and LB treatment. The above research showed that S3-1 strain could promote the accumulation of nutrients in protected vegetables. Its cells and fermentation broth could inhibit the accumulation of nitrate in vegetables in a short time,and increase the number

关 键 词：植物促生菌 AMOA基因 硝酸盐含量 土壤微生物群落 

分 类 号：S182[农业科学—农业基础科学]