‘上海双棱扁’大麦种子紫色色素以及脂质组分的研究  

Studies on purple pigment and lipid components of ’SLB’ barley seeds (Hordeum vulgare L.)

作　　者：赵慧燕 周龙华 刘成洪[2] 李锦斌 黄剑华[2] 陆瑞菊[2] ZHAO Huiyan;ZHOU Longhua;LIU Chenghong;LI Jinbin;HUANG Jianhua;LU Ruiju(Shanghai Ocean University,Shanghai 201306,China;Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China;Shanghai Haifeng Dafeng Seed Industry Co.,Ltd.,Yancheng 224100,China)

机构地区：[1]上海海洋大学,上海201306 [2]上海市农业科学院生物技术研究所,上海市农业遗传育种重点实验室,上海201106 [3]上海海丰大丰种业有限公司,盐城224100

出　　处：《上海农业学报》2023年第1期54-60,共7页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目[沪农科创字(2018)第1-2号];西藏自治区重大科技专项(XZ202101ZD0004N-02);现代农业产业技术体系专项。

摘　　要：以“紫籽”(purple-seeded,“PS”)以及“黄籽”(yellow-seeded,“YS”)大麦为研究材料,利用液相色谱-质谱联用仪(liquid chromatograph mass spectrometer,LC-MS)系统分析大麦籽粒表儿茶素(epicatechin,EC)含量。结果表明:“紫籽”大麦籽粒表儿茶素(epicatechin,EC)含量显著高于“黄籽”大麦。通过同源比对获得‘SLB’中Hv TTG1(TRANSPARENT TESTA GLABRA1)全长基因,在拟南芥中超表达Hv TTG1后,转基因拟南芥叶片颜色呈现紫色。脂质组学分析显示,“紫籽”中多种脂质组分含量均高于“黄籽”,包括1种单酰甘油(monoacylglyceride,MG)、12种二酰甘油(diacylglyceride,DG)、67种三酰甘油(triacylglyceride,TG)以及具有高营养价值的亚油酸(C18∶2)与亚麻酸(C18∶3)。综上,初步认为Hv TTG1在大麦种子紫色种皮形成中起到潜在的调控作用,并且‘紫籽’大麦相对‘黄籽’大麦,籽粒含有更高的亚油酸以及亚麻酸。研究可对紫色大麦在新食谱中的应用提供一定的参考依据。The contents of epicatechin(EC) in barley grain were analyzed by liquid chromatograph mass spectrometer(LC-MS) system using purple-seeded(“PS”) and yellow-seeded(“YS”) seeds were isolated from Shanghai local cultivar ‘Shanghai ShuangLengBian’(‘SLB’). The results showed that EC in “PS” barley grain was significantly higher than that in “YS” barley. The full-lengthHv TTG1(TRANSPARENT TESTA GLABRA1)gene in ‘ SLB’ was successfully cloned through homologous comparison. After overexpressingHv TTG1 in Arabidopsis,the purple pigments disappeared in leaves in transgenic lines. Lipidomics analysis showed that the contents of various lipid components in “ PS” were higher than those in “ YS”,including 1 monoacylglyceride(MG),12 diacylglycerides( DG),67 triacylglycerides( TG) and linoleic acid( C18 ∶ 2) and linolenic acid(C18∶ 3) with enhanced nutritional value. Therefore,this study preliminarily suggested thatHv TTG1 played a potential regulatory role in the formation of purple grains in barley seeds,and the grains of “PS” barley contained higher linoleic acid and linolenic acid than that of “YS” barley. This study provides a certain reference value for the application of purple barley in new recipes.

关 键 词：紫籽大麦 表儿茶素 脂质组学 液质联用 HvTTG1 

分 类 号：S512.3[农业科学—作物学]