卵黄在鸡精液冷冻保存中的应用研究  

Study on the application of yolk in chicken semen cryopreservation

作　　者：何孟纤 孙玲伟 戴建军[1] 汪俊跃 徐皆欢 吴彩凤[1] 张树山[1] 张德福[1] HE Mengqian;SUN Lingwei;DAI Jianjun;WANG Junyue;XU Jiehuan;WU Caifeng;ZHANG Shushan;ZHANG Defu(Institute of Animal Husbandry and Veterinary Science,Shanghai Academy of Agricultural Sciences,Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetics and Breeding,Shanghai 201106,China)

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106

出　　处：《上海农业学报》2023年第1期108-112,共5页Acta Agriculturae Shanghai

基　　金：国家重点研发计划资助(2021YFD1200300);上海市农业科学院助跑计划人才项目。

摘　　要：以Lake’s为基础稀释液,通过在鸡精液冷冻过程中添加全卵黄和不同浓度卵黄上清,探究卵黄及其离心处理在鸡精液冷冻中的应用效果。结果表明:与对照相比,稀释液中添加10%的全成分卵黄处理鸡精液冷冻保存后活率(22.33%)和活力(13.26%)显著下降(47.81%,42.55%),质膜完整性、顶体完整性和线粒体活性也下降显著。卵黄经稀释和离心处理后,添加5%-15%的卵黄上清可提高鸡精液的冷冻保存效果,其中以10%的卵黄上清效果最佳,其冻后活率和活力可达58.93%和55.36%,显著高于对照组。当卵黄上清添加浓度达到25%时,冻精质量开始明显下降。综上,在鸡精液冷冻过程中,添加全卵黄成分不利于其冷冻保存;10%的卵黄上清可显著提高鸡精子的抗冻能力。The application effects of egg yolk and its centrifugal treatment on rooster semen freezing were studied by adding whole yolk and different concentrations of egg yolk plasma in the Lake’s diluent. The results showed the viability(22. 33%),motility(13. 26%),acrosome integrity(10. 96%),plasma membrane integrity(9. 60%),mitochondrial activity(13. 73%) of the 10% whole egg yolk addition treatment were much lower than those of control. After dilution and centrifugation,the 5% -15% egg yolk plasma addition in diluent improved the freezing efficiency of rooster semen,and 10% egg yolk plasma group got the best freezing results,and its viability and motility got 58. 93% and 55. 36%,which were significantly higher than those of control. When the egg yolk plasma addition concentration reached 25%,the quality of rooster freezen semen began to decrease significantly. In conclusion,the addition of whole egg yolk was harmful to rooster semen freezing,while 10% egg yolk plasma addition after supernatant and centrifugation could significantly improve the anti-freezing ability of rooster sperms.

关 键 词：鸡 精液冷冻 卵黄 卵黄上清 

分 类 号：S831[农业科学—畜牧学]