两种饵料条件下点篮子鱼幼鱼酶活力及其消化道菌群比较  

COMPARISON OF DIGESTIVE TRACT FLORA AND ENZYME ACTIVITY OF JUVENILE SIGANUS GUTTATUS UNDER TWO FEEDING CONDITIONS

作　　者：孙艳秋 李琪[1,2] 刘鉴毅 庄平 杨俊[1,2] 彭彪彪[1,2] 秦搏 赵峰[1,2,3,4] 冯广朋 黄晓荣 SUN Yan-Qiu;LI Qi;LIU Jian-Yi;ZHUANG Ping;YANG Jun;PENG Biao-Biao;QIN Bo;ZHAO Feng;FENG Guang-Peng;HUANG Xiao-Rong(National Demonstration Center for Expermental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;East China Sea Fisheries Researsh Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;Key Scientific Observing and Experimental Station of Fisheries Resource and Environment in the Yangtze Estuary and East China Sea,Ministry of Agriculture and Rural Affairs,Shanghai 200090,China;Engineering Research Center of Fisheries Stock Enhancement and Habitat Restoration of the Yangtze Estuary,Shanghai 200090,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]中国水产科学研究院东海水产研究所,上海200090 [3]农业农村部东海与长江口渔业资源环境科学观测实验站,上海200090 [4]上海长江口渔业资源增殖和生态修复工程技术研究中心,上海200090

出　　处：《水生生物学报》2023年第3期495-504,共10页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划(2019YFD0900405);中国水产科学研究院东海水产研究所基本科研业务费(2021Z02);国家科技基础条件平台项目资助。

摘　　要：以点篮子鱼(Siganus guttatus)幼鱼为研究对象,分别用人工饲料和浒苔(Enteromorpha prolifera)进行饲喂,采用高通量测序技术结合生化分析方法,系统对比两种饵料对点篮子鱼(Siganus guttatus)幼鱼肝脏及消化道相关酶活力和消化道菌群的影响。结果显示,浒苔组点篮子鱼幼鱼虽然生长性能低于人工饲料组,但其淀粉酶活性更高,点篮子鱼对浒苔有良好的摄食和消化能力,浒苔足以满足点篮子鱼生长的需求;此外浒苔组点篮子鱼幼鱼消化道碱性磷酸酶、超氧化物歧化酶活性均显著高于人工饲料组,表现出了更高的免疫和抗氧化能力。在两种饵料条件下,菌群多样性随消化道延伸呈上升趋势,点篮子鱼幼鱼消化道菌群组成与饵料有关,变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)为共有优势菌门;浒苔组消化道菌群整体多样性较饲料组高,组成结构更加丰富多样,有利于鱼类的生长。研究结果表明点篮子鱼摄食浒苔具有良好的可行性基础,生态价值显著,值得深入研究与推广。Enteromorpha prolifera is a common large-scale alga,which can make filaments grow vertically in the pond in a short time(1—2 weeks).It is a common enemy organism in filter feeding shellfish culture ponds.Once it breaks out,it will not only affect the growth of cultured varieties,but also cause biological diseases and even endanger the survival of cultured varieties,resulting in economic losses.This paper attempts to make use of the habit of Siganus guttatus feeding on Enteromorpha prolifera,and compare Enteromorpha prolifera as a natural bait with artificial feed.In order to study the structure of digestive tract flora,digestive enzyme and nonspecific immune enzyme activity of juvenile Siganus guttatus were fed with artificial feed and Enteromorpha prolifera respectively.High-throughput sequencing technology combined with biochemical analysis methods were used to systematically analyze and compare the composition and distribution of microflora in the stomach,pyloric caecum and intestine of juvenile Siganus guttatus,and to compare the influence on growth performance and digestive enzyme activity of the juvenile Siganus guttatus with the two diets.Biochemical analysis showed that although the growth performance of juvenile Siganus guttatus fed with Enteromorpha prolifera was lower than that of the artificial feed group,its amylase activity was higher than that of the artificial feed group.The juvenile Siganus guttatus had good feeding and digestive ability to Enteromorpha prolifera,which was enough to meet the growth needs of Siganus guttatus.The activities of alkaline phosphatase and superoxide dismutase in the digestive tract of Enteromorpha prolifera group were significantly higher than those in the artificial diet group,showing higher immune and antioxidant capacity.High throughput sequencing results showed that bacterial diversity shows an upward trend with the extension of the digestive tract.The dominant bacteria in the digestive tract were Proteobacteria,Firmicutes and Bacteroidetes.The composition of bac

关 键 词：人工饲料 浒苔 消化道菌群 消化酶活性 非特异性免疫酶活性 点篮子鱼 

分 类 号：S965.3[农业科学—水产养殖]