太子参叶斑病菌 Phoma sp.FJZR01固体发酵代谢产物研究  

Metabolites of Phoma sp.FJZR01 from Pseudostellaria heterophylla Leaf Spot Pathogen on Solid Fermentation

作　　者：倪建成 范永飞 王泽榕 阮少江 陈美霞 叶祖云 NI Jian-cheng;FAN Yong-fei;WANG Ze-rong(Engineering Technology Research Center of Characteristic Medicinal Plants of Fujian/School of Life Sciences,Ningde Normal University,Ningde,Fujian 352100;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;Agricultural College,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)

机构地区：[1]福建省特色药用植物工程技术研究中心/宁德师范学院生命科学学院,福建宁德352100 [2]福建农林大学食品科学学院,福建福州350002 [3]福建农林大学农学院,福建福州350002

出　　处：《安徽农业科学》2023年第3期176-179,共4页Journal of Anhui Agricultural Sciences

基　　金：国家重点研发计划中药现代化专项(2019YFC1710500);中央引导地方科技发展专项资金项目(2021L3030);福建省自然科学基金项目(2020J05226);宁德师范学院科研项目(2017FZ04,2018Y14)。

摘　　要：为了解太子参叶斑病菌Phoma sp.FJZR01的固体发酵代谢产物,采用Sephadex LH-20柱色谱、RP-C_(18)柱色谱和正相硅胶柱色谱等方法对其进行分离鉴定,并应用叶片穿刺法测定化合物对太子参叶片的毒害作用。结果表明,从菌株FJZR01固体发酵代谢产物中分离得到6个化合物,结构鉴定分别为3-氯-4-羟基苯乙酸(1)、3-氯-4-羟基苯乙酰胺(2)、flemingipanic acid(3)、腺苷(4)、乙基α-D-吡喃葡萄糖苷(5)、甘露醇(6)。化合物1~6均是首次从太子参叶斑病菌中分离得到,化合物3系首次从微生物代谢产物中分离得到。3-氯-4-羟基苯乙酸(1)在1 mg/mL对太子参叶片造成明显的毒害作用,形成组织坏死状叶斑,与田间发病症状相似。目前尚未有关于3-氯-4-羟基苯乙酸(1)致病性相关报道,是一种新发现的真菌毒素。In order to understand the metabolites of solid fermentation of Pseudostellaria heterophylla leaf spot fungus Phoma sp.FJZR01,Sephadex LH-20 column chromatography,RP-C_(18) column chromatography and normal silica gel column chromatography were used to isolate and identify them,and the toxicity of compounds to Pseudostellaria heterophylla leaves was determined by leaf puncture method.The results showed that six compounds were isolated from the metabolites of solid fermentation of strain FJZR01,and their structures were identified as 3-chloro-4-hydroxyphenylacetic acid(1),3-chloro-4-hydroxyphenylacetamide(2),flemingipanic acid(3),adenosine(4),ethylα-D-glucopyranoside(5),mannitol(6).Compounds 1-6 were isolated from the leaf spot pathogen of Pseudostellaria heterophylla for the first time,while compound 3 was firstly isolated from microbial metabolites.At a dose of 1 mg/mL,3-chloro-4-hydroxyphenylacetic acid(1)caused obvious toxic effect on the leaves of Pseudostellaria heterophylla,resulting in leaf spots with tissue necrosis,similar to field symptoms of the disease.There was no relevant literature report on the pathogenicity of 3-chloro-4-hydroxyphenylacetic acid(1)for now,which was a newly discovered mycotoxin.

关 键 词：太子参 叶斑病菌 固体发酵代谢产物 3-氯-4-羟基苯乙酸 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]