Legiolert酶底物法结合质谱法对环境样本水体中嗜肺军团菌定量检测分离  

Quantitative detection and isolation of Legionella pneumophila in environmental water sample by Legiolert enzyme substrate method combined with mass spectrometry

作　　者：金萍[1] 葛藤 叶艳华[1] 斯佳丽 江晓[1] 张洪英[1] JIN Ping;GE Teng;YE Yan-hua;SI Jia-li;JIANG Xiao;ZHANG Hong-ying(Nanjing Center for Disease Control and Prevention,Nanjing,Jiangsu 210000,China)

机构地区：[1]南京市疾病预防控制中心,江苏南京210000

出　　处：《中国临床研究》2023年第2期281-285,共5页Chinese Journal of Clinical Research

基　　金：南京市卫生青年人才培养工程(QRX11127)。

摘　　要：目的探讨Legiolert酶底物法结合质谱法直接对环境样本水体中嗜肺军团菌定量检测分离的应用。方法采集2021年7月至11月南京14家公共场所,其中包括宾馆饭店、休闲中心等集中空调冷却水18份,淋浴水30份等共计48份样本。利用Legiolert酶底物法直接对环境样本水体中嗜肺军团菌定量检测,并对分离培养的纯菌落结合质谱法,荧光定量PCR试验确认后血清学分型,与传统分离培养法相比较得出两种方法对环境样本的嗜肺军团菌检出率。结果48份样本两种方法嗜肺军团菌检出率分别为8.33%(4/48)、31.25%(15/48),两种检测方法差异有统计学意义(χ^(2)=5.37,P<0.05)。结论Legiolert酶底物法结合质谱法对环境样本水体中嗜肺军团菌检出率明显高于传统分离培养法。Objective To investigate the application of Legiolert enzyme-substrate method with mass spectrometry in direct quantitative detection and separation of Legionella pneumophila in environmental water samples.Methods Collection of 14 public places in Nanjing from July 2021 to November 2021,including hotels and restaurants,leisure centers and other centralized air conditioning cooling water 18,shower water 30 and other total 48 samples.Legiolert enzyme-substrate method was used to detect Legionella pneumophila in water samples,and the isolated cultured pure colonies were identified for serological typing with mass spectrometry and fluorescence quantitative PCR test.The detection rate of Legionella pneumophila was compared with that by traditional isolated and cultured method.Results The detection rates of Legionella pneumophila in 48 samples were 8.33%(4/48)by traditional method and 31.25%(15/48)by mass spectrometry and fluorescence quantitative PCR.There was a significantly difference in it between two detection methods(χ^(2)=5.37,P<0.05).Conclusion Legiolert enzyme-substrate method combined with mass spectrometry shows obviously higher positive detection rate of Legionella pneumophila in environmental water samples compared with the traditional isolation and cultivation.

关 键 词：Legiolert酶底物法 质谱法 嗜肺军团菌 定量检测分离 

分 类 号：R126.4[医药卫生—环境卫生学]