泽泻醇提物对亚临床性甲状腺功能减退症母鼠甲状腺功能及仔鼠神经功能的作用及其机制  

Effect and mechanism of alcohol extract of Alisma orientalis on the thyroid function of mother rats and neurological function of offspring with subclinical hypothyroidism

作　　者：李燕君[1] 俄洛吉[1] 李玉琴[1] Li Yan-Jun;E Luo-Ji;Li Yu-Qin(Department of Obstetrics,Qinghai Provincial People's Hospital,Xining,Qinghai 810000,China)

机构地区：[1]青海省人民医院产科,青海西宁810000

出　　处：《解放军医学杂志》2023年第2期183-189,共7页Medical Journal of Chinese People's Liberation Army

基　　金：青海省科学技术基金(9632021Y0401)。

摘　　要：目的 探讨泽泻醇提物对亚临床性甲状腺功能减退症(SCH)母鼠甲状腺功能及仔鼠神经功能的作用及其可能的机制。方法 取85只8周龄SPF级Wistar雌鼠,其中15只行假手术处理作为假手术组,另70只采用甲状腺切除+皮下注射左旋甲状腺素(L-T4)制备SCH模型,造模成功62只,随机分为模型组(n=15,等体积生理盐水)、低剂量泽泻醇提物组(n=15,9 g/kg泽泻醇提物灌胃)、高剂量泽泻醇提物组(n=16,36 g/kg泽泻醇提物灌胃)、L-T4组(n=16,4.5μg/kg L-T4灌胃),1次/d,干预时间为妊娠10 d(E10)至仔鼠出生后21 d(P21)。E17时,采用电化学发光法检测母鼠血清促甲状腺素(TSH)、甲状腺素(T4)水平。P21时,ELISA法检测仔鼠海马组织神经生长因子(NGF)、脑源性神经营养因子(BDNF)水平,Westernblotting检测仔鼠海马组织原肌球蛋白受体激酶A(TrkA)、p-TrkA、环磷腺苷效应元件结合蛋白(CREB)、p-CREB蛋白表达水平。P40时,采用定位巡航实验测定仔鼠的逃避潜伏期;P41时,采用空间探索实验检测仔鼠穿越平台的次数。结果 E17时,5组母鼠的血清T4比较差异无统计学意义(P>0.05);低、高剂量泽泻醇提物组及L-T4组母鼠血清TSH水平明显低于模型组,高剂量泽泻醇提物组、L-T4组血清TSH水平明显低于低剂量泽泻醇提物组(P<0.05),而高剂量泽泻醇提物组与L-T4组血清TSH水平比较差异无统计学意义(P>0.05)。P21时,低、高剂量泽泻醇提物组及L-T4组仔鼠海马组织NGF、BDNF、p-TrkA、p-CREB水平明显高于模型组,高剂量泽泻醇提物组、L-T4组海马组织NGF、BDNF、p-TrkA、p-CREB水平明显高于低剂量泽泻醇提物组(P<0.05),而高剂量泽泻醇提物组与L-T4组海马组织NGF、BDNF水平比较差异无统计学意义(P>0.05)。低、高剂量泽泻醇提物组及L-T4组仔鼠逃避潜伏期短于模型组,穿越平台次数多于模型组(P<0.05);高剂量泽泻醇提物组、L-T4组仔鼠逃避潜伏期短于低剂量泽泻醇提物组,穿越�Objective To investigate the effect and possible mechanism of the alcohol extract of Alisma orientalis on thyroid function of mother rats and neurological function of offspring with subclinical hypothyroidism(SCH). Methods A total of 85 female rats were selected, of which 15 rats acted as sham operation group and treated with sham operation, the other 70 rats underwent thyroidectomy + subcutaneous injection of levothyroxine(L-T4) to establish the SCH model. Sixty-two of the 70 rats were successfully modeled, and randomly divided into low-dose Alisma ethanol extract group(15 rats, gavage with alcohol extract of Alisma orientalis 9 g/kg), high-dose Alisma ethanol extract group(16 rats, gavage with alcohol extract of Alisma 36 g/kg), L-T4group(16 rats, gavage with 4.5 μg/kg L-T4), model group(15 rats, gavage with equal volume of normal saline), once a day, the intervention time is from 10th day of pregnancy(E10) to 21st day after birth(P21). The thyroid stimulating hormone(TSH) and thyroxine(T4) of female rats at E17 were detected by electrochemiluminescence. ELISA was used to detect the nerve growth factor(NGF) and brain-derived neurotrophic factor(BDNF) in the hippocampus of offspring rats at P21;Western blotting was performed to detect the expression levels of tropomyosin receptor kinase A(TrkA), p-TrkA, c-AMP-response element binding protein(CREB)and p-CREB in the hippocampus of offspring rats at P21;The escape latency of pups at P40 was measured by the positioning cruise experiment, and the number of times the pups crossed the platform at P41 was measured by the space exploration experiment.Results At E17 gestational age, there was no significant difference in serum T4among the 5 groups of female mice(P>0.05). The serum TSH levels were lower in low-and high-dose Alisma ethanol extract group and L-T4group than that in model group, and the serum TSH levels were lower in high-dose Alisma ethanol extract group and L-T4group than that in low-dose Alisma ethanol extract group(P<0.05), while no significant difference

关 键 词：亚临床性甲状腺功能减退症 泽泻醇提物 甲状腺功能 神经功能 原肌球蛋白受体激酶A信号通路 

分 类 号：R965.1[医药卫生—药理学]