双酚A通过氧化应激干扰小鼠卵母细胞体外成熟  

Bisphenol A interfere with maturation of mouse oocytes in vitro through oxidative stress

作　　者：万辽 王爽[2] 于金红 丁婧如 邹清 王喜艳[2] Wan Liao;Wang Shuang;Yu Jin-Hong;Ding Jing-Ru;Zou Qing;Wang Xi-Yan(School of Public Health,Jilin Medical University,Jilin City,Jilin 132013,China;Morphology Laboratory,Jilin Medical University,Jilin City,Jilin 132013,China;Clinical Medical College,Jilin Medical University,Jilin City,Jilin 132013,China;College of Pharmacy,Jilin Medical University,Jilin City,Jilin 132013,China)

机构地区：[1]吉林医药学院公共卫生学院,吉林省吉林市132013 [2]吉林医药学院形态学实验室,吉林省吉林市132013 [3]吉林医药学院临床医学院,吉林省吉林市132013 [4]吉林医药学院药学院,吉林省吉林市132013

出　　处：《解放军医学杂志》2023年第2期198-203,共6页Medical Journal of Chinese People's Liberation Army

基　　金：吉林省大学生创新创业训练计划项目(201913706069)。

摘　　要：目的 观察双酚A(BPA)对小鼠卵母细胞体外成熟的影响,探讨相关的氧化应激损伤机制。方法 将雌性性成熟ICR小鼠的卵母细胞随机分为对照组、二甲基亚砜(DMSO)组和BPA组,对照组体外正常培养,DMSO组培养液中加入0.1%DMSO,BPA组培养液中加入溶于0.1%DMSO的45?μmol/L BPA。获取处于第二次减数分裂中期(MII)和停滞在生发泡期(GV)的小鼠卵母细胞。采用试剂盒检测MII期和GV期小鼠卵母细胞中活性氧(ROS)的水平;采用免疫荧光法和Western blotting法检测MII期和GV期小鼠卵母细胞中抗氧化酶的表达水平。结果 与对照组比较,体外成熟过程中接触45?μmol/L BPA的BPA组小鼠卵母细胞成熟率明显降低(26.32%±1.12%vs. 98.22%±0.89%,P<0.05),GV期和MII期卵母细胞中ROS水平明显升高(P<0.05),过氧化氢酶(CAT)和超氧化物歧化酶2(SOD2)水平明显降低(P<0.05)。结论 小鼠卵母细胞体外接触45?μmol/L BPA可增高ROS水平,抑制抗氧化酶CAT和SOD2的表达,引起氧化应激损伤,进而抑制卵母细胞体外成熟。Objective To investigate the effect of exposure to bisphenol A(BPA) on maturation and oxidative stress injury in mouse oocytes in vitro. Methods Oocytes from female ICR mice were randomly divided into control group, DMSO group,and BPA group. Mouse oocytes in control group was cultured normorly in vitro,while added 0.1% DMSO in DMSO group, and 45μmol/L BPA in 0.1% DMSO in BPA group. MII oocytes and oocytes stagnated in the GV phase were obtained.The levels of ROS in MII and GV oocytes of each group was detected by kit. The expression level of antioxidant enzyme in MII and GV oocytes was detected by immunofluorescence and Western blotting. Results Compared with control group, exposure to 45μmol/L BPA during in vitro maturation, resulted in a significant decrease in the maturation rate of mouse oocytes in BPA group(26.32%±1.12%vs. 98.22%±0.89%, P<0.05), the levels of ROS in GV and MII oocytes were significantly higher(P<0.05), the levels of antioxidant enzymes CAT and SOD2 in GV and MII oocytes were significantly decreased(P<0.05). Conclusion Exposure to 45μmol/L BPA increased ROS production and inhibited antioxidant enzyme expression, leading to oxidative stress damage of mouse oocytes and thus inhibiting oocyte maturation in vitro.

关 键 词：双酚A 卵母细胞 氧化应激损伤 

分 类 号：R332[医药卫生—人体生理学]