药食同源组方联合益生菌对免疫低下小鼠的免疫增强作用  

The Immune-enhancing Effect of Drug-food Homologous Prescription Combined with Probiotics on Immunocompromised Mice

作　　者：刘海春 胡晨旭 王家明 刘瑞 李敬华[4] LIU Haichun;HU Chenxu;WANG Jiaming;LIU Rui;LI Jinghua(Longkou Nanshan Health Valley Cancer Hospital&Nanshan Institute of Health Industry,Longkou,265718,China;College of Biotechnology,Tianjin University of Science&Technology&Tianjin Key Laboratory of Industry Microbiology,College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Tianjin Xiaowei Biological Technology Co.Ltd.,Tianjin 301799,China;Institute of Information on Traditional Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]龙口南山养生谷肿瘤医院,南山健康产业研究院,山东龙口265718 [2]天津科技大学生物工程学院,工业发酵微生物教育部重点实验室,天津300457 [3]天津小薇生物科技有限公司,天津301799 [4]中国中医科学院中医药信息研究所,北京100700

出　　处：《现代食品科技》2023年第2期119-124,共6页Modern Food Science and Technology

基　　金：国家自然科学基金项目(81502947);天津市教委基金项目(20140219);龙口南山养生谷肿瘤医院项目(20210906)。

摘　　要：该研究旨在探讨药食同源组方联合益生菌对环磷酰胺(CTX)诱导免疫低下小鼠免疫功能的调节作用。除空白对照组用生理盐水灌胃外,其余各组小鼠腹腔注射环磷酰胺(CTX)100 mg/kg(按小鼠体质量计),连续腹腔注射4 d。将建模成功体重相近的小鼠随机分为6组:正常对照组、免疫抑制组、药食同源组方Ⅲ号、药食同源改良组(Ⅳ号)、益生菌组(Ⅴ号)和Ⅳ和Ⅴ联用组(Ⅵ号)。连续灌胃给药14 d,观察Ⅲ、Ⅳ、Ⅴ和Ⅵ对小鼠胸腺指数、脾脏指数、吞噬能力、脾淋巴细胞增殖、NK细胞活性以及血清中IgG、IgA和IgM生成水平的影响。结果显示:Ⅲ、Ⅳ、Ⅴ和Ⅵ组均能显著提高模型小鼠胸腺指数(1.46、1.67、1.50、1.92 g/kg)、脾脏指数(2.32、2.54、2.52、2.82 g/kg)、吞噬能力(0.43、0.45、0.44、0.62)、脾淋巴细胞增殖作用(1.39、1.45、1.42、1.43)、NK细胞活性(0.35、0.43、0.36、0.71)(P<0.05);Ⅳ、Ⅴ和Ⅵ均能促使IgG(224.21、163.21、253.35 mg/L)、IgA(34.86、22.09、41.17 mg/L)和IgM(84.01、78.23、100.28 mg/L)生成水平均明显提高(P<0.05)。研究结果表明,药食同源改良组方与益生菌联用组对环磷酰胺致免疫低下小鼠的免疫能力具有显著增强作用。The aim of this study was to investigate the regulatory effect of the combination of drug-food homologous prescription and probiotics on the immune function of immunocompromised mice induced by cyclophosphamide(CTX).Except for the control group gavaged with normal saline,the mice in the other groups were intraperitoneally injected with CTX(100 mg/kg bw,for 4 days).Mice with similar body weights were randomly divided into 6 groups:control group,immunosuppression(model)group,drug-food homologous group Ⅲ,drug-food homologous improvement group(Ⅳ),probiotics group(Ⅴ),and Ⅳ-Ⅴ combination group(Ⅵ).After the administration for 14 days,the effects of Ⅲ,Ⅳ,Ⅴ and Ⅵ on the thymus and spleen indices,phagocytic ability,spleen lymphocyte proliferation,NK cell activity and the levels of the formed IgG,IgA and IgM in immunodepressed mice were assessed.The results showed that the thymus index(1.46,1.67,1.50 and 1.92 g/kg)and spleen index(2.32,2.54,2.52 and 2.82 g/kg),phagocytic ability(1.39,1.45,1.42 and 1.43),spleen lymphocyte proliferation(1.39,1.45,1.42 and 1.43)and NK cells activity(0.35,0.43,0.36 and 0.71)were enhanced significantly(P<0.05)in the group Ⅲ, Ⅳ, Ⅴ and Ⅵ. Ⅳ, Ⅴ and Ⅵ all significantly increased the levels of IgG(224.21,163.21 and 253.35 mg/L),Ig A(34.86,22.09 and 41.17 mg/L),and IgM(84.01,78.23 and 100.28 mg/L)(P<0.05).These results suggested that the combination of the modified drug-food homologous prescription and probiotics could enhanced significantly the immune function of the CTX-immunosuppressed mice.

关 键 词：药食同源组方 多肽 益生菌 免疫功能 

分 类 号：R285.5[医药卫生—中药学]