中国首次青春期前男孩睾丸组织冷冻保存3例报道并文献复习  

First testis cryopreservation of prepuberty boys in China:a report of 3 cases and literature review

作　　者：刘贵华[1] 马朦惠 苏文龙 颜禄斌[2] 孙德娟[1] 张靖[1] 李海涛 郑雅露 麦会思 赵鲁刚[1] 孙鹏[1] 曾海涛[1] 方丛[1] 王德娟 梁晓燕[1] Liu Guihua;Ma Menghui;Su Wenlong;Yan Lubin;Sun Dejuan;Zhang Jing;Li Haitao;Zheng Yalu;Mai Huisi;Zhao Lugang;Sun Peng;Zeng Haitao;Fang Cong;Wang Dejuan;Liang Xiaoyan(Center of Reproductive Medicine,the Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou 510655,China;Department of Pediactrics,the Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou 510655,China)

机构地区：[1]中山大学附属第六医院生殖中心,广州510655 [2]中山大学附属第六医院小儿外科,广州510655

出　　处：《中华生殖与避孕杂志》2023年第1期84-89,共6页Chinese Journal of Reproduction and Contraception

基　　金：国家自然科学基金(81971759,82171604)。

摘　　要：目的探讨重度β地中海贫血(β-thalassemia,β-TM)青春期前男孩在性腺毒性治疗前进行生育力保存的方法。方法报道3例重度β-TM男孩行造血干细胞移植(hematopoietic stem cell transplantation,HSCT)前,采用慢速程序化冷冻方法行睾丸组织冷冻保存,并结合文献对青春期前男孩进行生育力保存的必要性和地中海贫血男孩生育力监测、睾丸冷冻方法学及其相关流程进行阐述。结果采用慢速程序化冷冻方法分别成功为3例男孩冷冻31块、31块、20块睾丸组织,冷冻过程顺利。结论对拟行HSCT的重度β-TM男孩,青春期前睾丸组织冷冻可以保存其生育力,为其成年后孕育后代留下希望。Objective To explore the method of fertility preservation in severeβ-thalassemia prepubertal boys who cannot produce sperm before gonadotoxicity therapy.Methods Three cases of severeβ-thalassemia patients who were going to undergo hematopoietic stem cell transplantation(HSCT)were reported.They were received testis cryopreservation by slow-rate freezing.The necessity of fertility preservation in prepubertal boys and the methods of fertility persevation,testicular cryopreservation and the downstream techniques were stated.Results Totally 31,31,20 pieces of testicular tissue were frozen by slow-rate freezing in three boys respectively and the freezing process went smoothly.Conclusion The cryopreservation of testicular tissue can preserve the fertility of severeβ-thalassemia prepuberty boys who will receive HSCT,leaving hope for offspring.

关 键 词：Β地中海贫血 睾丸组织冷冻 慢速程序化冷冻 男性 生育力保存 

分 类 号：R714.8[医药卫生—妇产科学]