Structural insight into chitin perception by chitin elicitor receptor kinase 1 of Oryza sativa  

作　　者：Li Xu Jizong Wang Yu Xiao Zhifu Han Jijie Chai 

机构地区：[1]Tsinghua-Peking Center for Life Sciences,Beijing Advanced Innovation Center for Structural Biology,Centre for Plant Biology School of Life Sciences Tsinghua University,Beijing 100084 China [2]Max Planck Institute for Plant Breeding Research,Cologne 50829 Germany [3]Institute of Biochemistry University of Cologne,Cologne 50674 Germany [4]Cluster of Excellence in Plant Sciences(CEPLAS),Düsseldorf 40225 Germany

出　　处：《Journal of Integrative Plant Biology》2023年第1期235-248,共14页植物学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (31421001 to Jijie Chai and 31971119 and 32171193 to Zhifu Han);the Alexander von Humboldt Foundation (Humboldt professorship to Jijie Chai);the MaxPlanck-Gesellschaft (Max Planck fellowship to Jijie Chai),Deutsche Forschungsgemeinschaft (SFB-1403-414786233 to Jijie Chai);Germany’s Excellence Strategy CEPLAS (EXC-2048/1,Project 390686111 to Jijie Chai)。

摘　　要：Plants have developed innate immune systems to fight against pathogenic fungi by monitoring pathogenic signals known as pathogen-associated molecular patterns(PAMP)and have established endo symbiosis with arbuscular mycorrhizal(AM)fungi through recognition of mycorrhizal(Myc)factors.Chitin elicitor receptor kinase 1 of Oryza sativa subsp.Japonica(OsC ERK1)plays a bifunctional role in mediating both chitin-triggered immunity and symbiotic relationships with AM fungi.However,it remains unclear whether OsC ERK1 can directly recognize chitin molecules.In this study,we show that OsC ERK1 binds to the chitin hexamer((NAG)6)and tetramer((NAG)4)directly and determine the crystal structure of the OsC ERK1-(NAG)6complex at 2?.The structure shows that one OsC ERK1 is associated with one(NAG)6.Upon recognition,chitin hexamer binds OsC ERK1 by interacting with the shallow groove on the surface of LysM 2.These structural findings,complemented by mutational analyses,demonstrate that LysM 2 is crucial for recognition of both(NAG)6and(NAG)4.Altogether,these findings provide structural insights into the ability of OsC ERK1 in chitin perception,which will lead to a better understanding of the role of OsCERK1 in mediating both immunity and symbiosis in rice.

关 键 词：CHITIN immune response OsCERK1 recognition mechanism symbiotic reaction 

分 类 号：S511[农业科学—作物学]