石菖蒲挥发油对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白、c-Jun氨基末端蛋白激酶和肿瘤坏死因子α表达的影响  

Effects of volatile oil from Acori graminei Rhizoma on glial fibrillary acidic protein,c-Jun N-terminal protein kinase and tumor necrosis factor-αexpressions in the spinal cord dorsal horn of the inflammatory pain rats

作　　者：张润恒 杨翠珠 李诗琪 王姝涵 王欣 刘靖 马宇昕 ZHANG Run-heng;YANG Cui-zhu;LI Shi-qi;WANG Shu-han;WANG Xin;LIU Jing;MA Yu-xin(Department of Human Anatomy and Histology and Embryology,College of Life Sciences and Biopharmaceuticals,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广东药科大学生命科学与生物制药学院人体解剖学与组织学胚胎学系,广州510006

出　　处：《解剖学报》2023年第1期23-29,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金青年项目(81802678);广东省自然科学基金(2018A0303130073);广东省医学科学技术研究基金(B2022076)。

摘　　要：目的探讨石菖蒲挥发油(VOA)对炎性痛大鼠脊髓背角中胶质纤维酸性蛋白(GFAP)、c-Jun氨基末端蛋白激酶(JNK)和肿瘤坏死因子α(TNF-α)表达的影响。方法36只雄性SD大鼠随机分为:对照组(control)、假手术组(sham)、完全弗氏佐剂组(CFA)、5 g/(kg·d)低剂量VOA+CFA组(VOA-L+CFA)、10 g/(kg·d)中剂量VOA+CFA组(VOA-M+CFA)和20 g/(kg·d)高剂量VOA+CFA组(VOA-H+CFA),共6组,每组6只,于连续灌胃给药的第22天取材。利用免疫荧光染色和Western blotting技术检测各组大鼠脊髓背角中GFAP、JNK和TNF-α的表达情况。结果免疫荧光染色及Western blotting结果表明,与control和sham组相比,CFA组中大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达均显著增多(P<0.01);与CFA组相比,不同剂量VOA处理组大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达均减少,且VOA-H+CFA组大鼠脊髓背角中GFAP、JNK和TNF-α阳性表达减少更明显(P<0.05,P<0.01)。结论VOA可降低CFA诱导的炎性痛大鼠脊髓背角中GFAP、JNK和TNF-α的表达。Objective To investigate the influence of volatile oil from Acori graminei Rhizoma(VOA)on expressions of glial fibrillary acidic protein(GFAP),c-Jun N-terminal protein kainse(JNK)and tumour necrosis factor-α(TNF-α)in the spinal cord dorsal horn of imflammatory pain rats.Methods Totally 36 male SD rats were randomly divided into control group(control),sham-operated group(sham),complete Freund’s adjuvant group(CFA),5 g/(kg·d)low dose VOA+CFA group(VOA-L+CFA),10 g/(kg·d)medium dose VOA+CFA group(VOA-M+CFA)and 20 g/(kg·d)high dose VOA+CFA group(VOA-H+CFA).All animals were sacrificed immediately after continuous gavage administration for 22 days.The expressions of GFAP,JNK and TNF-αin the spinal cord dorsal horn of rats in each group were detected by immunofluorescence and Western blotting methods.Results The present results showed that the positive expressions of GFAP,JNK and TNF-αin the spinal cord dorsal horn of rats increased significantly in the CFA group,when compared to the control and sham groups(P<0.01).The expressions of GFAP,JNK and TNF-αin the spinal cord dorsal horn of rats with VOA treatment reduced in the dose-dependent manner,when compared to the CFA group,the positive expressions of GFAP,JNK and TNF-αreduced significantly in the dorsal horn of the spinal cord of the VOA-H+CFA group(P<0.05,P<0.01).Conclusion VOA reduces the expressions of GFAP,JNK and TNF-αin the spinal cord dorsal horn of rats of CFA-induced inflammatory pain.

关 键 词：石菖蒲挥发油 炎性痛 胶质纤维酸性蛋白 c-Jun氨基末端蛋白激酶 肿瘤坏死因子Α 免疫荧光 免疫印迹法 大鼠 

分 类 号：R338.22[医药卫生—人体生理学]