高脂血症大鼠脑缺血/再灌注后p38 MAPK活化及对Bax和Bcl-2表达的影响  

Activation of p38 MAPK and its effect on the expression of Bax and Bcl-2 after cerebral ischemia-reperfusion in hyperlipidemia rats

作　　者：高赛红[1] 张小良[1] 杨迎春[1] 乔海兵[1] GAO Sai-hong;ZHANG Xiao-liang;YANG Ying-chun;QIAO Hai-bing(Department of Anatomy,Fenyang College of Shanxi Medical University,Shanxi Fenyang 032200,China)

机构地区：[1]山西医科大学汾阳学院解剖学教研室,山西汾阳032200

出　　处：《解剖学报》2023年第1期50-55,共6页Acta Anatomica Sinica

基　　金：山西医科大学汾阳学院人才引进启动基金(2017D10)。

摘　　要：目的检测高脂血症大鼠脑缺血/再灌注(I/R)损伤后大脑皮质内磷酸化p38 MAPK(p-p38 MAPK)、Bax和Bcl-2表达变化,及SB203580对p-p38 MAPK、Bax和Bcl-2表达的影响,研究在高脂血症脑I/R损伤中p38 MAPK活化对Bax和Bcl-2表达的影响。方法大鼠高脂血症模型建立后,将其随机分为3组,假手术组(sham)、手术组(I/R)、SB203580处理组(SB+I/R),每组10只。线栓法建立左侧大脑中动脉栓塞I/R模型,神经行为学评分观察大鼠神经行为损伤症状,2,3,5-氯化三苯基四氮唑(TTC)染色显示脑梗死灶,TUNEL染色观察凋亡细胞,免疫组织化学法分析p-p38 MAPK、Bax和Bcl-2相对表达水平。结果与sham组比较,I/R组大鼠脑梗死体积百分比、细胞凋亡指数和神经行为学评分均显著升高,且p-p38 MAPK、Bax表达明显增高,Bcl-2表达明显降低,差异均具有统计学意义(P<0.05)。与I/R组比较,SB+I/R组大鼠脑组织损伤减轻,梗死灶明显缩小,细胞凋亡指数明显降低,p-p38 MAPK表达明显降低,Bax表达减少而Bcl-2表达增多,差异均有统计学意义(P<0.05)。SB+I/R组较I/R组神经行为学评分降低,但差异无统计学意义。结论在高脂血症大鼠脑缺血再灌注损伤过程中,p38 MAPK活化可调节Bax和Bcl-2的表达。Objective To detecte the expressions of phosphorylated p38 MAPK(p-p38 MAPK),Bax and Bcl-2 in the cerebral cortex of hyperlipidemia rats after cerebral ischemia-reperfusion(I/R)injury and the effect of SB203580 on the expressions of p-p38 MAPK,Bax and Bcl-2,to explore the effect of p38 MAPK activation on the expressions of Bax and Bcl-2 in hyperlipidemia cerebral I/R injury.Methods After the hyperlipidemia model was established,the rats were randomly divided into 3 groups:sham operation group,operation group(I/R)and SB203580 treatment group(SB+I/R),with 10 rats in each group.The focal cerebral I/R model in hyperlipemia rats was established with thread embolism of the left middle cerebral artery.The neurobehavioral score was used to observe the symptoms of neurobehavioral injury.The 2,3,5-triphenyltetrazolium chloride(TTC)staining was used to detect the volume of cerebral infarction,and the TUNEL staining was used to observe apoptotic cells.The relative expression levels of p-p38 MAPK,Bax and Bcl-2 were analyzed by immunohistochemistry.Results Compared with the sham group,the infarct volume,apoptosis index and neurobehavioral score of rats in the I/R group increased significantly,and the expressions of p-p38 MAPK and Bax increased significantly,and the expression of Bcl-2 decreased significantly(P<0.05).Compared with the I/R group,rats in the SB+I/R group had less brain damage,the infarct volume and the apoptosis index were significantly reduced,the expressions of p-p38 MAPK reduced significantly,Bax expression decreased while Bcl-2 expression increased.The differences were statistically significant(P<0.05).Neurobehavioral scores were lower in SB+I/R group than in I/R group,but the difference was not statistically significant.Conclusion In the process of cerebral I/R injury in hyperlipidemiarats,activation of p38 MAPK can regulate the expression of Bax and Bcl-2.

关 键 词：高脂血症 脑缺血/再灌注损伤 磷酸化P38丝裂原活化蛋白激酶 Bcl-2相关X蛋白 B淋巴细胞瘤-2 SB203580 免疫组织化学 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]