青海汉族妊娠期高血压疾病白细胞介素10基因启动子区-592A/C多态性分子特征  

Molecular analysis of interleukin 10 promoter region-592A/C polymorphism in hypertensive disorders of pregnancy in Qinghai Han nationality

作　　者：段倩 李建华 李文佳 王茹 武震 袁明 DUAN Qian;LI Jian-hua;LI Wen-jia;WANG Ru;WU Zhen;YUAN Ming(Department of Human Anatomy,Basic Medicine Science,Medical College of Qinghai University,Xining 810001,China)

机构地区：[1]青海大学医学院基础医学部人体解剖学教研室,西宁810001

出　　处：《解剖学报》2023年第1期63-69,共7页Acta Anatomica Sinica

基　　金：青海省科技厅项目(2019-SF-134)。

摘　　要：目的探讨白细胞介素10(IL-10)基因启动子区-592A/C(rs1800872)多态性与青海汉族妇女妊娠期高血压疾病(HDP)的相关性,并确定该基因在HDP组和健康对照组中的表达情况。方法选择青海省HDP患者(HDP组)和正常妊娠妇女(对照组)各140例,以血液DNA为模板,应用限制性内切酶片段长度多态性聚合酶链反应(PCR-RFLP)方法检测HDP组和对照组IL-10-592A/C多态性分型并测序验证。应用Real-time PCR检测两组胎盘组织中IL-10 mRNA的表达。应用ELISA检测两组血浆IL-10水平。结果HDP组和对照组IL-10基因AA、AC、CC基因型频率分别为24.29%、44.29%、31.42%和13.57%、41.43%、45.00%,两组基因型分布差异有统计学意义(P<0.05);AA基因型频率HDP组(24.29%)高于对照组(13.57%)(P<0.05),CC基因型频率HDP组(31.42%)低于对照组(45.00%)(P<0.05),而AC基因型频率两组间差异无统计学意义(P>0.05);IL-10-592A/C多态性A、C等位基因频率两组间分布有差异,HDP组A等位基因频率高于对照组(χ^(2)=8.079,P<0.05);HDP组胎盘组织中IL-10 mRNA表达量低于对照组(P<0.01);HDP组血浆IL-10水平(1.53±0.68)ng/L低于对照组(1.79±0.72)ng/L(P<0.01)。结论IL-10-592A/C多态性与青海汉族HDP相关;IL-10-592A/C多态性中A等位基因可能通过调节IL-10的表达参与HDP的遗传易感性。Objective To investigate the association between the interleukin 10(IL-10)gene promoter region-592A/C(rs1800872)polymorphism and hypertensive disorders of pregnancy(HDP)in Han women of Qinghai province and to determine the expression of this gene in two groups(HDP group and healthy control group)preliminarily.Methods A total of 140 HDP patients(HDP group)and 140 normal pregnant women(control group)in Qinghai Province were selected.Using blood DNA as template,the IL-10-592A/C polymorphism typing of HDP group and control group was detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)and verified by sequencing.The expression of IL-10 mRNA in the placental tissues of the two groups was detected by Real-time PCR.Plasma IL-10 levels of the two groups were detected by ELISA.Results The frequencies of AA,AC and CC genotypes of IL-10 gene in HDP group and control group were 24.29%,44.29%,31.42%and 13.57%,41.43%,45.00%respectively,the difference in genotype distribution between the two groups was statistically significant(P<0.05);AA genotype frequency in HDP group(24.29%)was higher than that of control group(13.57%)(P<0.05),CC genotype frequency in HDP group(31.42%)was lower than that in control group(45.00%)(P<0.05),while there was no significant difference in genotype frequency of AC between the two groups(P<0.05);The distribution of A and C allele frequencies of IL-10-592A/C polymorphism was different between the two groups,and the A allele frequency of HDP group was higher than that of control group(χ2=8.079,P<0.05);The expression of IL-10 mRNA in the placental tissue of the HDP group was lower than that of the control group(P<0.01);The level of plasma IL-10 in HDP group(1.53±0.68)ng/L was lower than that in control group(1.79±0.72)ng/L(P<0.01).Conclusion IL-10-592A/C polymorphism is associated with HDP in Qinghai Han nationality.The A allele in IL-10-592A/C polymorphism may be involved in the genetic susceptibility to HDP by regulating the expression of IL-10.

关 键 词：妊娠期高血压疾病 白细胞介素10 基因多态性 细胞因子 实时定量聚合酶链反应 限制性内切酶片段长度多态性聚合酶链反应 孕妇 

分 类 号：R3[医药卫生—基础医学]